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| siRNA抑制人前列腺癌细胞PC3的MDM2表达及细胞增殖 | |
| 引用本文： | 李然伟,赵燕颖,杨泽城,张舵舵,吕佳音,刘喜春. siRNA抑制人前列腺癌细胞PC3的MDM2表达及细胞增殖[J]. 肿瘤防治研究, 2009, 36(1): 24-27. DOI: 10.3971/j.issn.1000-8578.2009.01.007 |
| 作者姓名： | 李然伟 赵燕颖 杨泽城 张舵舵 吕佳音 刘喜春 |
| 作者单位： | 1. 吉林大学第二临床医院泌尿外科,长春,130041 2. 深圳市蛇口人民医院内科 3. 吉林大学中日联谊医院普外科 4. 吉林大学中日联谊医院放疗科 5. 吉林大学中日联谊医院骨科 |
| 基金项目： | 吉林省科委社会发展资助项目 |
| 摘 要： | 目的 研究siRNA MDM2对人前列腺癌细胞PC3的MDM2表达和细胞增殖的作用。 方法 构建PGCsilencerTM MDM2 siRNA，并转染入PC3细胞,分别用RT-PCR和 Western blot检测siMDM2对PC3细胞MDM2基因和蛋白表达的抑制作用；用MTT法检测siMDM2对PC3增殖抑制的作用，用流式细胞术检测siMDM2对PC3细胞凋亡的影响。 结果 PT-PCR和Western blot结果显示siMDM2能显著抑制PC3细胞MDM2基因和蛋白的表达，抑制率最高可达65%;MTT和流式细胞术结果证明siMDM2能显著抑制PC3细胞增殖并诱导细胞凋亡。 结论 siMDM2能特异有效地抑制MDM2在PC3中的表达，并抑制PC3细胞增殖，促进其凋亡。 |
| 关 键 词： | siRNA MDM2 前列腺癌 RT-PCR 流式细胞术 |
| 收稿时间： | 2007-07-12 |
| 修稿时间： | 2007-12-14 |
siRNA Inhibits MDM2 Expression and Cell Proliferation in PC-3 Cell Line |
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| LI Ran-wei,ZHAO Yan-ying,YANG Ze-cheng,ZHANG Duo-duo,LV Jia-yin,LIU Xi-chun. siRNA Inhibits MDM2 Expression and Cell Proliferation in PC-3 Cell Line[J]. Cancer Research on Prevention and Treatment, 2009, 36(1): 24-27. DOI: 10.3971/j.issn.1000-8578.2009.01.007 | |
| Authors: | LI Ran-wei ZHAO Yan-ying YANG Ze-cheng ZHANG Duo-duo LV Jia-yin LIU Xi-chun |
| Affiliation: | 1.Department of Urinary Surgery，The second Hospital of Jilin University，Changchun 130041，China； 2. Department of internal medicine, Shenzhen Shekou Peodle’s Hospital； 3. Department of Surgery, China Japan Union Hospital of Jilin University, 4. Department of Radiotherapy, 5. Department of Orthopedics |
| Abstract: | Objective To study the effect of siRNA to MDM2 on cell proliferation and MDM2 expression in prostate the cancer cell PC3. Methods PGCsilencerTM- MDM2 siRNA was constructed and transfected into the PC3 cells. MDM2 gene expression was detected by RT-PCR and protein expression was analyzed using western blot. The inhibitory effect on cell proliferation was determined by MTT assay. The cell apoptosis was observed by flow cytometer. Results The results of RT-PCR and western blot showed that the expression of MDM2 was inhibited in the siRNA transfected group and the highest inhibitory rate was 65%. The results of MTT and flow cytometer showed that siRNA could suppress the proliferation and induce apoptosis of PC3 cells. Conclusion siRNA of MDM2 could significantly inhibit MDM2 expression, the proliferation of PC3 cells and induce apoptosis of PC3 cells. |
| Keywords: | siRNA MDM2 RT-PCR |
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