分枝杆菌培养中同步筛检依赖利福平结核分枝杆菌(依R菌)的临床应用价值

目的 分析探讨培养分枝杆菌时同步筛检依赖利福平结核分枝杆菌(依R菌)临床意义及价值. 方法 2011年3月—2015年3月所采集530例临床标本进行培养使用利福平浓度100μg/mL罗氏(L-J)培养基建立依R菌筛查系统,将待分离培养分枝杆菌平行接种于罗氏对照空白培养基(L-J)与L-J R100培养基中分别培养,依照培养基选用种类分别分成L-J R100、L-J、BD960 3组, 并对结果 进行分析统计. 结果 L-J R100培养基检出76例阳性结核分枝杆菌,且皆为依R菌,菌株粗大,生长良好,L-J R100阳性菌株所占比例为14.34%,L-J阳性菌株所占比例23.21%, BD960阳性菌株所占比例为52.83%. 结论 培养分枝杆菌时同步筛查依R菌能得到绝对依赖菌株,可提高分枝杆菌阳性检出率,可最快速度判断阳性菌株耐药性及是否对利福平具依赖性,该方法 可靠易行,具有临床推广价值.

Clinical Application Value of Synchronous Screening of Rifampicin-depen-dent Mycobacterium Tuberculosis During the Mycobacterium Culture Pro-cess

Objective To study the clinical significance of synchronous screening of Rifampin-dependent mycobacterium tuberculosis during the mycobacterium culture process. Methods From March 2011 to March 2015, 530 cases of clinical specimens were cultured, L-J medium with 100μg/mL of Rifampicin was applied to build up the screening system of Ri-fampicin-dependent mycobacterium tuberculosis. The isolated mycobacterium was separately cultured in L-J and L-J R100 medium. According to the selection of culture medium, the species were divided into three groups, L-J R100, L-J and BD960. The results were analyzed statistically. Results For L-J R100 medium, there were 76 positive cases with mycobac-terium tuberculosis. They were featured as Rifampin-dependent mycobacterium tuberculosis, large strains and favorable growth. The percentage of R100 L-J positive strains was 14.34%, the proportion of L-J positive strains was 23.21%, and the proportion of BD960 positive strains was 52.83%. Conclusion During the mycobacterium culture process, the synchronous screening of Rifampicin-dependent mycobacterium tuberculosis can identify the absolute strain, improve the positive detec-tion rate of mycobacterium, quickly identify the tolerance of positive train and its dependency on Rifampicin. The reliable method is worthy of clinical promotion.