米力农对LPS 诱导下人冠状动脉内皮细胞炎症反应的影响

目的:观察米力农对LPS 诱导下人冠状动脉内皮细胞(HCAEC)炎症反应及NF-κB 的影响。方法:体外培养HCAEC,分为正常组、LPS 组(1 μg/ ml)、米力农50 μmol/ L 组和米力农200 μmol/ L 组,后两组均与LPS 共同孵育24 h。分别采用qRT-PCR 和ELISA 检测IL-6、IL-8、TNF鄄琢的mRNA 和蛋白水平,分别采用Western blot 和免疫荧光法检测NF-κB 活化和核移位。结果:与LPS 组相比,米力农显著降低HCAEC 的IL-6、IL-8、TNF-α的mRNA 和蛋白表达;抑制NF-κB 活化和核移位(P<0.05),且呈一定量效关系。结论:米力农可通过抑制NF-κB 活化而降低LPS 诱导的HCAEC 释放炎症因子。

Effects of milrinone on LPS induced inflammation of human coronary artery endothelial cells

Objective:To observe the effects of milrinone on LPS-induced inflammation and NF-κB activation of human coronary artery endothelial cells (HCAEC).Methods: HCAEC were cultured in vitro,and were divided into normal group,LPS group (1 μg/ ml),milrinone 50 μmol/ L group and milrinone 200 μmol/ L group,the rear two groups were co-incubated with LPS for 24 h.qRT-PCR and ELISA was applied to detect IL-6,IL-8,TNF-αmRNA and protein,respectively.Western blot and immunofluorescence assay was used to observe the activation and translocation of NF-κB,respectively.Results: Compared with LPS group,the IL-6,IL-8,TNF-αmRNA and protein;the activation and translocation of NF-κB significantly inhibited after incubation with milrinone (P<0.05) in a dose dependent manner.Conclusion: Milrinone inhibited the release of inflammative cytokines induced by LPS of HCAEC in vitro,partly by inactivation of NF-κB。