CD46、Nectin-4 在麻疹病毒感染人肺泡上皮细胞中的作用

目的:探讨CD46、Nectin-4 在麻疹病毒感染人肺泡上皮细胞中的作用。以及CD46 和Nectin-4 之间的相互作用。方法:分为麻疹病毒感染前处理组和感染后2 h 组,均用抗CD46 抗体、抗Nectin-4 抗体处理人肺泡上皮细胞,并设对照组。检测细胞内病毒滴度的变化,采用Western blot 和Real-time PCR 检测NP 蛋白的表达水平,并运用免疫共沉淀检测CD46与Nectin-4 的相互作用。结果:与对照组相比,麻疹病毒感染前用抗CD46 抗体、抗Nectin-4 抗体处理的人肺泡上皮细胞内麻疹病毒滴度显著降低,分别为对照组的48.03% 和49.53%,同时用抗CD46 抗体、抗Nectin-4 抗体处理使病毒滴度下降为27.15%,差异均有统计学意义(P<0.01)。然而,在麻疹病毒感染2 h 后用抗CD46 抗体、抗Nectin-4 抗体处理,人肺泡上皮细胞内麻疹病毒滴度和NP 蛋白量未见显著减少。Western blot 和Real-time PCR 结果与上述结果一致;免疫共沉淀实验显示,CD46 与Nectin-4 存在相互作用。结论:CD46、Nectin-4 在麻疹病毒感染肺泡上皮细胞中起到介导作用,CD46 与Nectin-4 可能存在协同作用,该作用对麻疹病毒的感染起到了增强效果。

Role of CD46 and Nectin-4 on Measles virus (MV) infecting human pulmonary alveolar epithelial cells

Objective:To investigate the role of CD46 and Nectin-4 on Measles virus (MV) infecting human pulmonary alveolar epithelial cells (HPAEpiC),and the interaction between CD46 and Nectin-4.Methods: Measles virus was divided into pre-infection group and 2 h-infection group,HPAEpiCs treatment with anti-CD46 antibody and/ or anti-Nectin-4 antibody as experimental groups,and untreated HPAEpiCs as a control.The variation of viral replication level was detected.A Co-immunoprecipitation assay (Co-IP) was used to explore whether CD46 and Nectin-4 had interactive relationship in MV infection.Results: Compared with the control group,MV titers were reduced in HPAEpiCs of the pre-infection group treated with anti-CD46 and anti-Nectin-4 respectively (48.03%and 49.53%) .Furthermore, virus titers showed a more reduction in which treated with anti-CD46 and anti-Nectin-4 antibodies(27.15%,P<0.01).Western blot and Real-time PCR showed that anti-CD46 antibody and anti-Nectin-4 antibodies decreased the rate of MV infection.In the 2 h-infection group,however,the treatment with anti-CD46 and anti-Nectin-4 could significantly reduce the MV titer and NP protein in HPAEpiCs.The Co-IP assay showed that there were interaction between CD46 and Nectin-4.Conclusion: CD46 and Nectin-4 mediated MV infecting HPAEpiCs.Moreover,CD46 and Nectin-4 may play a synergetic role in MV infection,which could enhance the infection effect.