HLA-DQB1 基因多态性与广西壮族女性HPV16 感染和宫颈癌易感性的关联研究

目的:探讨HLA-DQB1 等位基因多态性对广西壮族女性HPV16 感染和宫颈癌发生的影响,为寻找广西壮族女性宫颈癌的遗传易感基因或保护基因提供线索。方法:选取广西地区25 ~45 岁宫颈癌确诊的壮族患者、无癌健康壮族女性各171 例作为研究对象(按年龄依3 岁配对),采集研究对象样本并提取HPV 核酸和人基因组DNA,分别应用PCR-SSP 和分子导流杂交技术进行HLA-DQB1 基因型检测和HPV 基因分型检测,最后进行统计学分析。结果:(1)171 例宫颈癌患者中HPV总感染率为91.22%,其中高危型病毒占90.76%, HPV16 型为主要致病亚型(43.58%);(2)广西壮族女性宫颈癌患者组的HLA-DQB1*04 等位基因携带率高于无癌对照组,差异具有统计学意义(P<0.05);等位基因HLA-DQB1*06/09 在宫颈癌患者组中的携带率明显低于无癌组,差异具有统计学意义(P<0.05);而两组间的HLA-DQB1*02/05/07/08 等位基因携带率无显著性差异(P>0.05);(3)HLA-DQB1*04 基因在HPV16 阳性宫颈癌患者中出现的频率明显高于HPV16 阴性患者,差异具有统计学意义(P<0.05)。结论:HLA-DQB1*04 可能是广西壮族女性宫颈癌发生的易感基因,HLA-DQB1*06/09 可能是广西壮族女性宫颈癌的保护基因。而HLA-DQB1*02/05/07/08 等位基因可能与广西壮族女性宫颈癌遗传易感性无关。携带HLA-DQB1*04 等位基因的广西壮族妇女可能更容易感染HPV16 型病毒,从而增加了其患宫颈癌的危险性。

Relationship between HLA-DQB1 allele polymorphism with HPV16 infection and cervical cancer susceptibility in Guangxi Zhuang women

Objective:To study the relationship of Guangxi Zhuang women being infected by HPV16 and suffering from cervical cancer with HLA-DQB1 allele polymorphism.Provide clues for seeking hereditary susceptibility gene or resistant gene of cervical cancer of Guangxi Zhuang women.Methods:Chose the cervical cancer diagnosed female patients and health women 171 cases respectively aged between 25 and 45 of Guangxi as subject investigated(people in the two groups were paired by age ±3 years).Took their samples to extract HPV DNA and human genome DNA.Then detected HLA-DQB1 alleles and HPV genetype applying PCR-SSP and molecular diversion hybrid technology.Finally the data were statistically analyzed.Results:(1)The total infection rate of HPV in 171 cases of cervical cancer patient was 91.22%,in which the high-risk virus accounted for 90.76%,HPV16 was the main pathogenic subtypes (43.58%).(2)The allele carrying rate of HLA-DQB1*04 in the cervical cancer group was higher than the health control group with statistically significant difference(P<0.05).The allele carrying rate of HLA-DQB1*06/09 in the cervical cancer group was lower than the health control group with statistically significant difference(P<0.05).There was no significant difference of the allele carrying rate of HLA-DQB1*02/05/07/08 between two groups(P>0.05).(3)The occurrence frequency of HLA-DQB1*04 alleles in HPV16 positive cervical cancer patients was significantly higher than HPV16 negative patients with statistically significant difference(P <0.05). Conclusion:HLA.DQB1*04 alleles are probably the susceptibility genes of cervical cancer of Guangxi Zhuang women;HLA-DQB1*06/09 alleles are probably the protective genes of cervical cancer of Guangxi zhuang women; HLA-DQB1*02/05/07/08 alleles seem irrelevant to hereditary susceptibility of cervical cancer of Guangxi Zhuang women.And Guangxi Zhuang women carried HLA-DQB1*04 alleles are more likely to infect HPV16 that increase the risk of cervical cancer.