长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响

目的：探讨长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响情况。方法：qPCR检测肺癌和癌旁组织、不同肺癌细胞中H19的表达情况；Transwell侵袭实验检测沉默H19后肺癌细胞侵袭能力的变化；划痕实验检测沉默H19后肺癌细胞迁移能力的变化；双荧光素酶报告基因检测H19与miR-107的相互作用；qPCR检测肺癌和癌旁组织、不同肺癌细胞中miR-107的表达情况；Transwell侵袭实验检测沉默H19后miR-107对肺癌细胞侵袭能力的影响；划痕实验检测沉默H19后miR-107肺癌细胞迁移能力的影响；裸鼠皮下成瘤检测沉默H19后miR-107对肺癌成瘤大小以及体积的影响。Western blot检测沉默H19后Notch通路蛋白的表达情况。结果：与癌旁组织相比，肺癌组织中H19表达明显增高；肺癌细胞A549中H19表达水平最高；沉默H19可以抑制肺癌细胞侵袭和迁移能力；H19能与miR-107的3′UTR特异性结合；与癌旁组织相比，肺癌组织中miR-107表达明显降低；沉默H19后，抑制miR-107可以促进肺癌细胞侵袭和迁移能力；与H19-siRNA组相比，H19-siRNA+miR-107-inhibitor组荷瘤小鼠肿瘤体积和重量都明显增大。沉默H19后Notch通路蛋白表达情况相应下调。结论：H19在肺癌发生发展过程中起重要作用，H19可以靶向调节miR-107通过Notch信号通路调控肺癌细胞的侵袭和迁移能力。

Long non-coding RNA H19 promoted lung cancer cells migration and invasion through miR-107

Objective:To investigate the effect and the related mechanism of H19 on the invasion and migration ability of pancreatic cancer cells.Methods: The expression of H19 in tissues of pancreatic carcinoma and non-carcinoma adjacent tissues of pancreatic carcinoma were detected.qPCR was used to detect the expression of H19 in different pancreatic cancer cells.The migration and invasion ability of pancreatic cancer cells was detected after silencing H19 using wound healing assays and Transwell matrigel invasion assays.Dual-Luciferase Reporter Assay System was used to detect miR-107regulating H19.The expression of miR-107 in tissues of pancreatic carcinoma and non-carcinoma adjacent tissues of pancreatic carcinoma were detected.The regulation of miR-107 on the migration and invasion ability of pancreatic cancer cells were detected after silencing H19 using wound healing assays and Transwell matrigel invasion assays.Subcutaneous tumor was used to detect the size and volume of the tumor after inject the tumor cells.Immunohistochemistry was used to detect the expression of Ki67 and PCNA.Results: Compared with non-carcinoma adjacent tissues of pancreatic carcinoma,the expression of H19 of tissues of pancreatic carcinoma was significantly increased.The expression of H19 in pancreatic cancer cell A549 was highest.Silencing H19 could inhibit the migration of human pancreatic cancer A549 cells.miR-107 was the direct target of H19.Compared with non-carcinoma adjacent tissues of pancreatic carcinoma,the expression of H19 of tissues of pancreatic carcinoma was significantly decreased.After silencing H19,inhibiting the expression of miR107 could inhibit the migration of human pancreatic cancer A549 cells.Compared with the group of H19-siRNA,H19-siRNA+miR-107-inhibitor group mice tumor volume and weight were significantly bigger.Immunohistochemistry showed that compared with H19-siRNA group,the expression Ki67 and PCNA expression in H19-siRNA+miR-107-inhibitor group increased.Conclusion: H19 plays the role of tumor promotor factor in pancreatic cancer.H19 can affect the invasion and migration ability of pancreatic carcinoma cells by regulating miR-107.