一种以虾原肌球蛋白为致敏原的Th2 反应小鼠模型的建立

目的:采用虾原肌球蛋白为致敏原建立Th2 反应小鼠模型。方法:虾原肌球蛋白腹腔注射小鼠6 周诱导Th2反应,ELISA 测定小鼠血清总IgE、sIgE 和sIgG 水平、脾淋巴细胞分泌Th1 和Th2 细胞因子的含量,采用流式细胞术检测血液中嗜碱性粒细胞的活化。结果:与对照组比较,致敏6 周的小鼠血清总IgE、sIgE 和sIgG(sIgG1、sIgG2a 和sIgG2b)均显著升高;脾淋巴细胞在抗原刺激后分泌Th2 细胞因子(IL-4、IL-5、IL-10 和IL-13)增加,而Th1 细胞因子INF-α则出现明显降低;血液中嗜碱性粒细胞表面标志物CD200R 和CD41 表达增强。结论:成功建立一种以虾原肌球蛋白为致敏原的Th2 反应小鼠模型。

A murine model of Th2 response induced by shrimp tropomyosin

Objective:To develop murine models of Th2 response induced by shrimp tropomyosin (ST).Methods: Mice were sensitized with ST for 6 weeks.The serum antigen-special IgE (sIgE),total IgE and sIgG level,Th1/ Th2 cytokines production were measured by ELISA.The basophil activation in mice was measured by flow cytometry.Results: The intraperitoneal sensitization with ST for 6 weeks induced significant increase of serum sIgE,total IgE and sIgG (sIgG1,sIgG2a and sIgG2b) level in mice.Th2 cell response was induced and cytokines (IL-4,IL-5,IL-10 and IL-13) production increased in splenocytes stimulated by ST,while Th1 cytokine (IFN-α) production decreased.As the markers of basophil activation,CD200R and CD41 expression also increased in response to ST.Conclusion: The Th2 response is dominant in ST-induced anaphylaxis in mice.