miR-126 对结肠癌细胞生物学行为的影响及其在调控结肠癌EMT 转化中的作用

目的:观察miR-126 在不同转移潜能的人结肠癌细胞系中的表达情况及其对结肠癌细胞增殖、侵袭转移能力的影响并探讨可能的作用机制。方法:采用实时荧光定量PCR 检测结肠癌细胞系(SW480、SW620 及HCT116)中miR-126 的表达量。通过脂质体瞬时转染法将miR-126 过表达(miR-126 mimics),并设置阴性对照组,然后采用CCK8 法检测细胞的增殖能力,细胞划痕实验检测细胞的迁移能力,Transwell 侵袭小室实验检测细胞的侵袭能力,Western blot 实验检测E-cadherin 和Vimentin 蛋白表达量的变化。结果:相对于低转移潜能的结肠癌细胞株SW480,miR-126 在高转移潜能的SW620 和HCT116细胞中的表达降低。过表达miR-126 可使SW620 细胞增殖、迁移和侵袭能力降低,E-cadherin 蛋白表达增加,Vimentin 蛋白表达降低,差异具有统计学意义(P<0.05)。结论:低表达的miR-126 与结肠癌的转移密切相关,miR-126 影响结肠癌细胞生物学行为的作用可能是通过调控EMT 进程实现的。

Role of miR-126 in effect of biological behaviour and epithelial to mesenchymal transition (EMT) in human colon cancer cells

Objective:To investigate the expression of miR-126 in human colon cancer cell lines with different metastatic potential and its effect on the proliferation, invasion and metastasis of colon cancer cells, and to explore the possible mechanism.Methods: The expression of miR-126 in human colon cancer cell lines (SW480,SW620 and HCT116) was determined by Real-time fluorescence quantitative PCR.miR-126 mimics were transiently overexpressed in SW620 cells throuIgh liposome transfection and the negative control group was set up.The proliferation ability of cells was detected by CCK8 method and the mobility of cells was detected by wound healing assay and Transwell migration and invasion assay.The expression of E-cadherin,Vimentin was determined by Western blot.Results: The expression of miR-126 was decreased in SW620 and HCT116 cells with high metastatic potential compared SW480 cells with low metastatic potential.The overexpression of miR-126 significantly inhibited the proliferation,migration and invasionability of SW620 cells and Western blot indicated that miR-126 overexpression increased the expression of E-cadherin and decreased the expression of Vimentin in SW620 cells,which was significantly different from that of negative control(P<0.05). Conclusion: Low expression of miR-126 is closely related to metastasis of colon cancer and the effect of miR-126 on the biological behavior of colon cancer cells may be mediated by the regulation of the EMT process.