HIV感染者T细胞NKG2C/NKG2A表达及与疾病进展关系研究

目的：研究HIV慢性感染者和HARRT治疗者T细胞NKG2C/NKG2A受体的表达变化，探讨其与疾病进展的关系。方法：选取HIV慢性感染者、接受HAART治疗的HIV感染者以及健康人的外周血细胞，通过荧光抗体染色，利用流式细胞仪检测T细胞上表达的NKG2C/NKG2A受体。结果：HIV慢性感染者NKG2C⁺ T细胞，NKG2A⁺ T细胞和NKG2C+NKG2A- T细胞百分比明显高于健康对照组 (P=0.025、P=0.032、P=0.029)，HARRT治疗组则明显低于HIV慢性感染者(P=0.033、P=0.037、P=0.018)，恢复到正常水平，与健康对照组相比无统计学差异。HIV慢性感染者外周血CD4⁺ T淋巴细胞绝对数和表达NKG2A、NKG2C⁺NKG2A⁺和NKG2C-NKG2A⁺ 的T细胞呈负相关(r=-0.697,P<0.000 1；r=-0.463,P=0.015；r=-0.693,P<0.000 1)，HIV慢性感染者外周血T细胞上表达的NKG2C与NKG2A的比值与CD4⁺ T淋巴细胞绝对数呈正相关(r=0.476,P=0.012)。结论：HIV感染者T细胞表面NKG2C和NKG2A的表达研究具有重要意义，为HIV感染的临床预后评估提供科学依据。

Research on expression of NKG2C/NKG2A receptors on T cells in HIV infected individuals and relationship with disease progression

Objective:To study the changes of NKG2C/NKG2A expressed on T cells in HIV chronically infected individuals and HAART-treatment AIDS patients and the relationship with disease progression of HIV.Methods: We collected peripheral blood from HIV chronically infected individuals,HAART-treatment AIDS patients and healthy human and used the flow cytometry by staining fluorescent antibody to detect the NKG2C/NKG2A receptors expressed on T cells.Results: NKG2C⁺,NKG2A⁺ and NKG2C⁺NKG2A^- expressed on T cells in HIV chronically infected individuals were significantly higher than the healthy control group (P=0.025，P=0.032，P=0.029),while in HAART-treatment AIDS patients were significantly lower than that in HIV chronically infected individuals (P=0.033，P=0.037，P=0.018),returned to the normal levels with no significant difference compared with the healthy control group.The absolute number of peripheral blood CD4⁺ T lymphocytes in HIV chronically infected individuals was negative correlation with T cells which expressing NKG2A⁺，NKG2C⁺NKG2A⁺ and NKG2C-NKG2A⁺ (r=-0.697,P<0.000 1；r=-0.463,P=0.015；r=-0.693,P<0.000 1).What was more,the absolute number of peripheral blood CD4⁺ T lymphocytes had positive correlation with the ratio of NKG2C and NKG2A expressed on T cells receptor in HIV chronically infected individuals(r=0.476,P=0.012).Conclusion: Studying the expression of NKG2C and NKG2A receptors on T cell has great significance in HIV infected individuals,which may provide a scientific basis for clinical prognosis of HIV infection.