噻可拉林协同顺铂促进宫颈癌c4-1及Hela细胞凋亡机制的研究

目的：探讨噻可拉林协同顺铂促进宫颈癌c4-1、Hela细胞凋亡的机制。方法：用不同浓度噻可拉林和顺铂单独或联合作用于体外培养的c4-1、Hela细胞，MTT法检测c4-1、Hela细胞生长抑制率，Hoechst 33342 和AO-EB染色法检测细胞凋亡，Western blot检测细胞Bax、Bcl2、p53、p21蛋白的表达，流式细胞术检测细胞增殖周期。结果：c4-1及Hela细胞经不同浓度的噻可拉林和顺铂单独或联合作用后，细胞的体外增殖活力被明显抑制，呈剂量依赖关系，且在联合使用噻可拉林（10 nmol/L）：顺铂（4 μmol/L）用量时抑制效果最明显（P<0.05）；AO-EB染色法显示，噻可拉林和顺铂联合使用，细胞凋亡更明显。Western blot则显示，Bax、p53、p21表达明显上调，而Bcl2的表达则明显下调。结论：噻可拉林能协同顺铂上调Bax、p53、p21的表达和下调Bcl2的表达，抑制宫颈癌c4-1、Hela细胞增殖同时促进细胞凋亡。

Mechanism of thiocoraline combination with cisplatin on promoting apoptosis of cervical cancer cell line c4-1 and Hela

Objective:To investigate the apoptosis by thiocoraline in combination with cisplatin on cervical cell line c4-1 and Hela and its mechanism. Methods: Different concentrations of thiocoraline and cisplatin alone or in combination used for cultured c4-1 and Hela cells.The inhibition of cell proliferation was detected by MTT assay,and the apoptosis was detected by Hoechst 33342 and AO-EB staining.The expressions of the protein Bax,Bcl2,p53,p21 were detected by Western blot.The cell cycle of c4-1 and Hela cells were detected by Flow cytometry.Results: The cell proliferation activity of c4-1 and HeLa cells were inhibited obviously by different concentrations of thiocoraline and cisplatin alone or in combination.The inhibition effect was in a dose-dependent manner.Meanwhile,when the concentrations of thiocoraline and cisplatin were 10 nmol/L and 4 μmol/L,respectivity the inhibitory effect was most significant (P<0.05);Hoechst 33342 and AO-EB staining showed that the cell apoptosis effect was more obvious on thiocoraline in combination with cisplatin.The Western blot showed that the expressions of the protein Bax,p53,p21 were significantly up-regulated,and the Bcl2 was significantly down-regulated.Conclusion: Thiocoraline combination with cisplatin could up-regulate the expression of the protein Bax,p53,p21 and down-regulation the expression of Bcl2 to inhibition the proliferation and promotion apoptosis of cervical cell line c4-1 and Hela.