miR-346 在鼻咽癌中的表达及生物学功能 |
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引用本文: | 蒋成义,汪洪涛,江,涛,许亚佳,夏,林.miR-346 在鼻咽癌中的表达及生物学功能[J].中国免疫学杂志,2017,33(6):833. |
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作者姓名: | 蒋成义 汪洪涛 江 涛 许亚佳 夏 林 |
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摘 要: | 目的:探讨miR-346 在鼻咽癌中的表达及在鼻咽癌中的生物学功能。方法:收集63 例鼻咽癌组织以及34 例
鼻咽部非癌组织标本,Real-time PCR 检测组织和6 株人鼻咽癌细胞系及1 株人正常鼻咽部永生化上皮细胞株NP69 中miR-
346 表达量,选取人鼻咽癌细胞系中表达量居中的两种细胞系,并转染miR-346 inhibitor,设置对照组(NC 组)并转染阴性对照
质粒,Real-time PCR 检测两种细胞系转染miR鄄346 inhibitor 和对照组miR鄄346 表达量,细胞增殖、凋亡分别采用Brdu-ELISA 和
流式细胞术检测,Transwell 小室实验检测细胞迁移和侵袭。结果:与鼻咽非癌组织比较,鼻咽癌组织中miR-346 表达量显著升
高(P =0.000);以正常人鼻咽部上皮细胞NP69 比较,各鼻咽癌细胞株中miR-346 相对表达量均显著升高,差异有统计学意义
(P<0.05)。选择6 种鼻咽癌细胞株中miR-346 表达量居中的两种,分别为CNE-1 和CNE-2,转染miR-346 inhibitor 后,两种鼻
咽癌细胞株miR-346 相对表达量均显著降低,与NC 组比较差异均有统计学意义(P<0.05)。两种鼻咽癌细胞转染miR-346
inhibitor 下调miR-346 表达后鼻咽癌细胞的增殖受到抑制,均在转染第3 天差异具有统计学意义(P<0.05)。下调miR-346 表
达后鼻咽癌细胞CNE-1 和CNE-2 的凋亡显著增加,迁移细胞数和侵袭细胞数显著降低,与NC 组比较差异均有统计学意义
(P<0.05)。结论:miR-346 在鼻咽癌中高表达,下调miR-346 表达会抑制鼻咽癌细胞增殖、迁移和侵袭,促进凋亡,miR-346 可
能作为一种癌基因在鼻咽癌的发生和发展中发挥重要的作用。
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关 键 词: | miR-346 鼻咽癌 增殖 凋亡 迁移 侵袭 |
Expression and biological functions of miR-346 in nasopharyngeal carcinoma |
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Abstract: | Objective:To explore the expression and biological functions of miR-346 in nasopharyngeal carcinoma.Methods:
63 cases nasopharyngeal carcinoma tissue and 34 cases nasopharyngeal non-cancer tissues were collected,the miR-346 expression were
detected by Real-time PCR between nasopharyngeal carcinoma tissue and nasopharyngeal non-cancer tissues,6 strains of human naso-
pharyngeal carcinoma cell lines and 1 strain of normal nasopharyngeal epithelial cell immortalized NP69, two strains of human
nasopharyngeal carcinoma cell lines whose miR-346 expression were middle were chose,and were with miR-346 inhibitor transfection,
the control group (NC group) were with negative control plasmid transfection,miR-346 expression in two groups were detected by Real-
time PCR,the proliferation,apoptosis were detected by Brdu-ELISA and flow cytometry,the migration and invasion were detected by Tr-
answell Chambers experiments.Results: Compared with the nasopharyngeal non-cancer tissues, the miR-346 expression in
nasopharyngeal carcinoma tissues was significantly increased (P = 0.000);Compared with the normal nasopharyngeal epithelial cells
NP69,the miR-346 expression in human nasopharyngeal carcinoma cell lines was significantly increased (P<0.05).The CNE-1 and
CNE-2 were chose,after the miR-346 inhibitor transfection,the miR-346 expression were significantly lower compared with NC group,
the difference was statistically significant (P<0.05).The proliferation of two kinds of nasopharyngeal carcinoma cell CNE-1 and CNE-2
were restrained after transfection,the difference showed statistically significant 3 days after transfection (P <0.05).The apoptosis
increased significantly,and the migration cell numbers and invasion cell numbers decreased significantly compared with NC group,the
differences were statistically significant(P<0.05). Conclusion:miR-346 is overexpression in nasopharyngeal carcinoma,down-regu
lation of miR-346 inhibits the proliferation,migration,invasion,promotes the apoptosis,miR-346 may act as a oncogene and play an
important role in the pathogenesis of nasopharyngeal carcinoma. |
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