TRAP1 通过TGF / Smad3 通路对膀胱癌的迁移和侵袭能力的影响

目的：探讨TRAP1蛋白对膀胱癌细胞迁移和侵袭的影响及机制。方法：采用Western blot从膀胱癌细胞株中挑选出高表达TRAP1的细胞系BIU-87；使用TRAP1沉默慢病毒（LV3-TRAP1）沉默TRAP1，使用GFP荧光及PCR检测LV3-TRAP1沉默效率；Transwell和划痕实验分别检测沉默TRAP1蛋白表达后对膀胱癌细胞侵袭和迁移能力的影响；CM-H2DCFDA荧光染色细胞检测膀胱癌细胞内活性氧水平；Western blot检测相关信号通路TGF/Smad3蛋白的表达情况。结果：LV3-TRAP1慢病毒可以有效地抑制TRAP1蛋白的表达；沉默TRAP1蛋白的表达可以有效抑制膀胱癌细胞的侵袭和迁移能力、降低膀胱癌细胞内活性氧水平；同时TGF/Smad3信号通路中的TGF、Smad2及Smad3蛋白表达也相应降低。结论：沉默TRAP1蛋白可以通过调控TGF/Smad3信号通路来抑制膀胱癌细胞的侵袭和迁移能力。

Effect of TRAP1 on invasion and migration of human bladder cancer through TGF/Smad3 signal pathway

Objective:To investigate the effect and related mechanism of TRAP1 on the invasion and migration of human bladder cancer through TGF/Smad3 signal path.Methods: Selected from BIU-87 of high expression of TRAP1 in bladder cancer cell lines through Western blot techniques.TRAP1 knockdown lentivirus (LV3-TRAP1) was used to silence the expression of TRAP1.GFP fluorescene and PCR detector was used to detected the efficiency of gene silencing and the effectiveness of gene silencing;effect of TRAP1 on the invasion and migration ability of BIU-87 were detected by Transwell matrigel invasion assays and wound healing assays,CM-H2DCFDA fluorescent staining was used to deteced the cell ROS of BIU-87 with LV3-TRAP1.Detected the level of TGF/Smad3 signal protein by Western blot.Results: LV3-TRAP1 lentivirus could effectively inhibit the expression of TRAP1 compared with LV3-NC.LV3-TRAP1 lentivirus could effectively inhibit the cell RPS of BIU-87.Knockdown the expression of TRAP1 could inhibit the invasion and migration of BIU-87.Knockdown the expression of TRAP1 in BIU-87 could reduce the protein level of TGF/Smad3.Conclusion: Silencing TRAP1 could inhibit the invasion and migration of bladder cancer cell through TGF/Smad3 signal pathway.