| 高效液相色谱法测定盐酸雷洛昔芬含量 |
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| 引用本文： | 王倩,张红梅,于治国. 高效液相色谱法测定盐酸雷洛昔芬含量[J]. 沈阳药科大学学报, 2002, 19(2): 105-108 |
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| 作者姓名： | 王倩 张红梅 于治国 |
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| 作者单位： | 1. 沈阳药科大学药学院,辽宁,沈阳,110016
2. 阜新第二制药厂,辽宁,阜新,123000 |
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| 摘 要： | 目的用高效液相色谱法测定盐酸雷洛昔芬的含量。方法采用ODS柱 ,流动相为乙腈 0 0 1mol/L十二烷基硫酸钠溶液 (体积比 5 5∶4 5 ,冰醋酸调节 pH值至 4 0 ) ,在波长2 86nm检测。结果测定了盐酸雷洛昔芬的含量 ,平均含量为 99 9% (RSD =0 4 6 % ,n =3)。样品进样量在0 5～ 2 5 μg内 ,与峰面积具有良好的线性关系 ,线性回归方程为 :A =-0 31 + 2 0 3 0 3·W ,r =0 9997。结论本测定方法灵敏、准确 ,重现性好 ,适用于盐酸雷洛昔芬及其制剂的常规分析。
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| 关 键 词： | 盐酸雷洛昔芬 高效液相色谱法 含量测定 |
| 文章编号： | 1006-2858(2002)02-0105-04 |
| 修稿时间： | 2001-06-12 |
Determination of the content of raloxifene hydrochloride by HPLC |
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| WANG Qian ,ZHANG Hong mei ,YU Zhi guo. Determination of the content of raloxifene hydrochloride by HPLC[J]. Journal of Shenyang Pharmaceutical University, 2002, 19(2): 105-108 |
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| Authors: | WANG Qian ZHANG Hong mei YU Zhi guo |
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| Affiliation: | WANG Qian 1,ZHANG Hong mei 2,YU Zhi guo 1 |
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| Abstract: | Objective To develop a reversed phase high performance liquid chromatographic method to determine the content of raloxifene hydrochloride. Method The mobile phase consisted of acetonitrile 0 01 mol/L sodium dodecyl sulfate (55∶45,adjusted to pH4 0 using glacial acetic acid ) and the UV absorbance was monitored at 286 nm . Result The average content was 99 9% with RSD=0 46%. Conclusion This assay demonstrated that the method had adequate sensitivity and selectivity to measure the concentrations of raloxifene hydrochloride and could be used in the quality control of raloxifene hydrochloride. |
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| Keywords: | raloxifene hydrochloride RP HPLC determination |
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