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| 大鼠骨髓间充质干细胞对免疫耐受的作用 | |
| 引用本文： | 王劲松,秦晓晔,舒振波,宋岩.大鼠骨髓间充质干细胞对免疫耐受的作用[J].中国免疫学杂志,2008,24(12). |
| 作者姓名： | 王劲松 秦晓晔 舒振波 宋岩 |
| 作者单位： | 1. 吉林省中医院,长春,130021 2. 吉林大学中日联谊医院,长春,130031 3. 长春中医药大学,长春,130117 |
| 摘 要： | 目的:体外分离培养大鼠骨髓间充质干细胞(Bone Marrow Mesenchymal Stem Cells,BMSCs),观察和检测其生长特性及表面标记,探讨它对免疫耐受的作用.方法:通过密度梯度离心、贴壁筛选法分离培养大鼠 BMSCs,观察细胞形态学变化,应用流式细胞术测定其表面分子 CD34、CD44 的表达.、将培养第 3 代的 BMSCs 经尾静脉输注大鼠,应用流式细胞技术检测大鼠外周血中CD4+CD25+Treg 比率,通过3H-TdR 掺入实验检测 BMSCs 对大鼠胸腺细胞自发增殖反应的影响.结果:体外培养的大鼠 BMSCs 呈贴壁生长,似成纤维细胞样,表面分子 CD44 表达阳性率为98%,但 CD34 表达阴性.输注 BMSCs 的大鼠外周血中CD4+CD25+Treg 占2.5%±O.69%,明显高于对照组(0.8%±0.14%)和PBS输注组(1.0%±0.23%),P<0.05;胸腺细胞白发增殖活性(cpm值3 275.41±1 736.05)明显低于正常对照组(7 368.25±2 532.13)和PBS输注组(6 143.71±2 380.47),P |
| 关 键 词： | 骨髓间充质干细胞 CD4^＋CD25^＋Treg 胸腺细胞自发增殖 免疫抑制 |
Effect of mesenchymal stem cells from rat bone marrow on immunological tolerance |
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| WANG Jin-Song,QIN Xiao-Ye,SHU Zhen-Bo,SONG Yan.Effect of mesenchymal stem cells from rat bone marrow on immunological tolerance[J].Chinese Journal of Immunology,2008,24(12). | |
| Authors: | WANG Jin-Song QIN Xiao-Ye SHU Zhen-Bo SONG Yan |
| Institution: | WANG Jin-Song,QIN Xiao-Ye,SHU Zhen-Bo,SONG Yan.JiLin Provincial Hospital Of Traditional Chinese Medicine,ChangChun 130031,China |
| Abstract: | Objective:To investiate biological properties of Mesenchymal stem cell(BMSC) from rat bone marrow in vitro,and their effects in inducing immune tolerance.Methods:BMSCs were obtained from rat bone marrow by density gradient centrifugation and selected by cell attachment.The morphology of BMSCs was observed by electron microscope.The expression of surface molecules of CD34 and CD44 was analyzed by flow cytometry.The cells were injected ex vivo intravenously after in vitro culture and then CD4+ CD25+ Treg rati... |
| Keywords: | CD4 CD25 Treg |
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