NF-κB、I-κB、Bcl-2在宫颈癌组织中的表达及其与人乳头瘤病毒感染的关系

背景与目的:已表明核因子κB(nuclearfactorκB,NF-κB)及其抑制蛋白κB(inhibitorproteinκB,I-κB)对凋亡基因的调控起重要作用,NF-κB在某些肿瘤中高表达,与肿瘤的发生有关,但在宫颈癌中的表达以及与人乳头瘤病毒(humanpapillomavirus,HPV)感染的关系未见报道。本研究旨在探讨宫颈癌组织中NF-κB、I-κB、bcl-2的表达及其与HPV感染的关系。方法:采用免疫组织化学SP法对46例宫颈癌和26例正常宫颈组织进行NF-κB、I-κB、bcl-2蛋白检测,用PCR技术对组织标本的HPV-DNA进行检测。结果:NF-κB、bcl-2在宫颈癌组织中的表达率分别为60.9%(28/46)和52.2%(24/46),在正常对照组的表达率分别为23.1%(6/26)和0(0/26),宫颈癌组显著高于正常对照组(P<0.01);I-κB在宫颈癌组和正常对照组的表达率分别为30.4%(14/46)和57.7%(15/26),宫颈癌组显著低于正常对照组(P<0.05);NF-κB与bcl-2的表达呈显著性相关(P<0.05)。HPV-DNA阳性的宫颈癌组织中NF-κB的阳性率显著高于阴性组(P<0.05);I-κB、bcl-2的阳性率在HPV-DNA阳性和阴性组之间无显著性差异(P>0.05)。结论:NF-κB、bcl-2的高表达和I-κB的低表达与宫颈癌的发生过程可能有关;HPV感染可能与NF-κB表达有关。

Correlation of expression of nuclear factor kappaB, inhibitor protein kappaB, and Bcl-2 in cervical cancer to human papillomavirus infection

BACKGROUND & OBJECTIVE: It was proved that nuclear factor kappaB (NF-kappaB) and its inhibitor protein kappaB (I-kappaB) played critical roles in regulating the expression of proapoptotic genes, and NF-kappaB is overexpressed in some tumors and related with tumorigenesis. However, the expression of NF-kappaB and I-kappaB in cervical cancer and its correlation to human papillomavirus (HPV) infection have not been reported yet. This study was to explore the correlation of the expression of NF-kappaB, I-kappaB, and Bcl-2 in cervical cancer to human papillomavirus (HPV) infection. METHODS: The expression of NF-kappaB, I-kappaB, and Bcl-2 were detected by immunohistochemistry in 46 specimens of cervical cancer and 26 specimens of normal cervical tissue. HPV DNA was detected by polymerase chain reaction (PCR). RESULTS: The positive rates of NF-kappaB and Bcl-2 were significantly higher in cervical cancer than in normal cervical tissue (60.9% vs. 23.1%, 52.2% vs. 0.0%, P < 0.01). The positive rate of I-kappaB was significantly lower in cervical cancer than in normal cervical tissue (30.4% vs. 57.7%, P < 0.05). The expression of NF-kappaB was closely related to Bcl-2 (P < 0.05). The positive rate of NF-kappaB was significantly higher in HPV DNA-positive cervical cancer than in HPV DNA-negative cervical cancer (81.3% vs. 35.7%, P < 0.05), but the positive rates of I-kappaB and Bcl-2 between these 2 groups had no significant difference (P >0.05). CONCLUSION: The high expression of NF-kappaB and Bcl-2 and the low expression of I-kappaB may be related to cervical carcinogenesis, and NF-kappaB expression may be related to HPV infection.