| 三个品系转基因小鼠的遗传监测 |
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| 引用本文： | 谢建云,邵伟娟,顾卫忠,王晓东,高诚. 三个品系转基因小鼠的遗传监测[J]. 实验动物与比较医学, 2001, 21(3): 147-149,T001 |
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| 作者姓名： | 谢建云 邵伟娟 顾卫忠 王晓东 高诚 |
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| 作者单位： | 1. 上海市实验动物质量监督检验站,上海,200032
2. 上海西普尔-必凯实验动物有限公司,上海,200237 |
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| 基金项目： | 上海市科技发展基金资助项目(9984119044) |
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| 摘 要： | 为了解转基因小鼠在繁育过程中其遗传特性的稳定情况，用特异性引物以PCR方法分别对IL－4T、IL－10T、IFNgR%三个品系转基因小鼠进行了遗传监测。结果表明，三个品系的转基因小鼠中的部分小鼠其整合基因发生了不同程度的改变与丢失。
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| 关 键 词： | 转基因小鼠 PCR 遗传监测 品系 |
| 文章编号： | 1004-8448(2001)03-0147-03 |
Genetic Monitoring on Three Strains of Transgenic mouse |
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| XIE Jian yun,SHAO Wei juan,GU Wei zhong,WANG Xiao dong,GAO Cheng. Genetic Monitoring on Three Strains of Transgenic mouse[J]. Laboratory Animal and Comparative Medicine, 2001, 21(3): 147-149,T001 |
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| Authors: | XIE Jian yun SHAO Wei juan GU Wei zhong WANG Xiao dong GAO Cheng |
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| Abstract: | Three strains of transgenic mouse(IL 4T?IL 10T?IFNgR%) were monitored by PCR technique with specific primers in order to assure the genetic quality of the three strains transgenic mouse after a long period propagation. The results showed that the recombined genes of part of the transgenic mouse were changed or lost. |
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| Keywords: | Transgenic mouse PCR Genetic monitoring |
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