白念珠菌保护性单抗的研究

目的 探讨抗白念珠菌单克隆抗体在系统性念珠菌感染动物中的保护作用。方法 制备抗白念珠菌单抗，观察单抗对系统性念珠菌感染小鼠存活时间，组织病理改变以及组织中菌落形成单位的影响。结果 制备出3株抗白念珠菌胞壁外膜抗原单克隆抗体-1B5、3E8、4C7；1B5、3E8两株单抗能显著延长致死量白念珠菌感染小鼠存活时间，减少感染小鼠主要脏器组织中白念珠菌菌落形成单位，减轻组织病理改变；1B5单抗能识别白念珠菌胞壁外膜上相对分子质量约为32000抗原；并在体外能抑制白念珠菌孢子对人颊粘膜上皮细胞，胎儿脐静脉内皮细胞的粘附。结论 1B5、3E8是具有保护作用的抗白念珠孢子对人颊粘膜上皮细胞，胎儿脐静脉内皮细胞的粘附。结论 1B5、3E8是具有保护作用的抗白念珠菌单抗；其中1B5是抗白念珠菌胞壁外膜上相对分子质量为32000的抗原的单抗；1B5单抗可通过抑制白念珠菌对上皮细胞，内皮细胞的粘附，降低该菌的侵袭力。

Protective Monoclonal Antibody Against Candida albicans: an Experimental Study

Objective To study the protective effect of mon oclonal antibodies(McAb)against Can-dida albicans in systemic candidiasis.Methods Monoclonal antibody was produced wi th hybridoma technique.The effect of McAb on experimental mo use systemic candidiasis was observ ed,expecially on the following as-pects:the survival time of mice,colony forming unit(CFU)of Candida albicans and the histopathologic changes.Results Three types of McAb against the cell w all antigen of Candida albicans were generated,which were designated as 1B5,3E8and 4C7.P rolonged survival time,decreased C FU of Candida albicans in kid-neys,liver and brain,and alleviate d histopathologic changes could be s een in experimental mice treated wit h McAb 1B5and 3E8.McAb 1B5could recog nize a cell wall component of Candida albicans(MW:32000),and inhibit the adherence of Candida albicans to human buccal epithelial cells and umbilical vein endothelial cells.Conclusion McAbs 1B5and 3E8,are the protective monoclonal antibodies against Candida albicans,which inhibit the adherence of Candida albicans to epithelial cells and endothelial cells,thus reducing the invasiveness of the pathogen.