重组胶原矿化骨负载骨形态发生蛋白2活性多肽复合材料的制备及其异位成骨研究

目的 构建重组胶原矿化骨/BMP2活性多肽新型仿生骨修复材料,并评价其异位成骨能力.方法 将48只成年SD大鼠随机分为4组,在A、B、C组的大鼠背部肌肉内分别植入含3、2、1 nag BMP2活性多肽的重组胶原矿化骨;在D组的大鼠背部肌肉内植入单纯重组胶原矿化骨,于4、8和12周时间点将大鼠处死,经CT三维重建、组织学观察及钙含龟测定检测植入材料的成骨情况.结果 A、B.C组,在各时间点的CT三维重建、组织学观察及钙含量检测结果均表明其异位成骨能力优于D组,差异有统计学意义(P<0.01),其中A、B两组的异位成骨能力优于C组,差异有统计学意义(P<0.01).结论 BMP2活性多肽町以显著增强重组胶原矿化骨的骨诱导活性,这种骨诱导性存在一定的剂量效应关系.

Preparation of composite of bone based mineralized recombinant collagen loading with BMP2-derived peptide and study on its ectopic osteogenesis

Objective To prepare a new biomimetie bone matrix material with BMP2-derivedpeptide combined with bone based mineralized recombinant collagen and investigate its eetopic osteogenetic capacity. Methods Forty-eight adult Sprague-Dawley rats were divided into four groups randomly. Muscle of the rats' back in group A,group B and group C was implanted with bone based mineralized recombinant collagen combined with 3,2,1 mg BMP2-derived peptide, separately. On the week 4,8 and 12 after implantation,the rats were killed and the samples were harvested. Their osteogenic capability was detected by three-dimensional reconstruction of computed tomography, histological observation and content determination of calcium. Results Results Results of three-dimensional reconstruction of computed tomography, histological observation and content determination of calcium indicated that the osteogenic capability of group A,group B and group C was superior to group D with the difference being statistically significant ( P <0.01 ). The osteogenic capability of group A and group B was superior to group C with the difference being statistically significant ( P <0.01 ). Conclusion BMP2-derived peptide can increase the osteoinduction of bone based mineralized recombinant collagen and the osteoinduction has dose-effect relation to some extent.