层粘连蛋白在金黄地鼠颊囊癌形成中的表达

目的检测了层粘连蛋白(LN)在金黄地鼠颊囊癌发生、发展过程中的表达,探索LN与颊囊癌侵袭性的关系。方法金黄地鼠60只,随机分成6组,1～5组用0.5％二甲基苯蒽涂擦金地鼠颊囊,每周3次,分别于6、8、10、12及14周处死。免疫组化法观察颊囊病变中LN的表达。结果轻、中、重度不典型增生原位癌中LN表达呈连续、线状;侵袭癌中LN则模糊、消失;侵袭前沿LN表达缺失;分化好的癌巢周围LN呈连续、线状。结论 LN可作为口腔癌侵袭性诊断的指标,可反映癌细胞的分化程度,可直观、准确地对原位癌进行诊断。

The expression of laminin in the course golden hamster buccal pouch carcinogenesis

Objective In order to investigate the relation between LN and the invasion of buccal pouch carinoma,the LN's expression in the course of golden hamster buccal pouch carcinogenesis had been detected.Methods 60 golden hamster were divided into 6 groups randomy,buccal pouch mucosas of 1-5 groups were applied with 0.5% DMBA,3 times a week,each group of animals were separatly killed at 6w,8w,12w and 14w after applied with DMBA,the normal group of animals were killed at 14 w.The buccal pouch samples were taken and fixed with 10% formalin,the sections were stained by immunohistological method (S-P method).Results LN expressed intact and continuous line in mild,moderate and esvere dysplasia/carcinoma in situ; it is ambiguous and lost in invasive carcinoma; it is disappeared at the front of invasion; LN encircled the well differentiated carcionma nests.Conclusion LN can be worked as a diagnostic index of oral carcinma's invasion,it can reflect the differentiated degree of cancer cell,LN's expression can diagnose carcinoma in situ accuratly.