| 人血管性血友病因子A1区单克隆抗体的研制及其生物学功能的初步研究 |
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| 引用本文： | 沈飞,董宁征,谢丽倩,范磊,赵益明,阮长耿. 人血管性血友病因子A1区单克隆抗体的研制及其生物学功能的初步研究[J]. 苏州大学学报(自然科学版), 2008, 28(2): 234-237 |
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| 作者姓名： | 沈飞 董宁征 谢丽倩 范磊 赵益明 阮长耿 |
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| 作者单位： | 苏州大学附属第一医院,江苏省血液研究所,卫生部血栓与止血重点实验室,江苏苏州215006 |
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| 摘 要： | 目的 研制人血管性血友病因子(vWF)A1区的单克隆抗体及其生物学特性的鉴定.方法 采用基因重组技术体外表达人vWF A1区蛋白(rv, WF-A1),以rvWF-A1免疫Balb/c小鼠,通过细胞融合,HAT选择性培养,用ELISA筛选阳性杂交瘤细胞.采用Western blot和免疫组织化学方法进行单抗鉴定.结果 构建了rvWF-A1表达载体pQE30-vWF-A1.rvWF-A1在大肠杆菌M15中稳定表达.通过融合、筛选获得两株阳性杂交瘤细胞,命名为SZ-129和SZ-130.其分泌的抗体能特异性地与rvWF-A1和人血浆中vWF结合.结论 表达了rvWF-A1,并制备了具有潜在抗血栓作用的vWF-A1单克隆抗体SZ-129和SZ-130.
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| 关 键 词： | 血管性血友病因子A1区 原核表达 血小板 单克隆抗体 人血管性血友病因子 单克隆抗体 生物学功能 研究 Domain von Willebrand Factor Human Monoclonal Antibodies Characterization 抗血栓作用 稳定表达 结合 人血浆 体能 命名 融合 大肠杆菌 表达载体 结果 单抗 |
| 文章编号： | 1673-0399(2008)02-0234-03 |
| 修稿时间： | 2007-04-10 |
Preparation and Characterization of Monoclonal Antibodies Against Human von Willebrand Factor A1 Domain |
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| SHEN Fei,DONG Ning-zheng,XIE Li-qian,FAN Lei,ZHAO Yi-ming,RUAN Chang-geng. Preparation and Characterization of Monoclonal Antibodies Against Human von Willebrand Factor A1 Domain[J]. Suzhou University Journal of Medical Science, 2008, 28(2): 234-237 |
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| Authors: | SHEN Fei DONG Ning-zheng XIE Li-qian FAN Lei ZHAO Yi-ming RUAN Chang-geng |
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| Affiliation: | SHEN Fei,DONG Ning-zheng,XIE Li-qian,FAN Lei,ZHAO Yi-ming,RUAN Chang-geng (The First Hospital Affiliated to Suzhou University,Jiangsu Institute of Hematology,Key Laboratory of Thrombosis , Haemostasis,Ministry of Health,Jiangsu Suzhou 215006,China) |
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| Abstract: | Objective To prepare monoclonal antibody(McAb) against human von Willebrand factor A1(vWF-A1)domain and characterize their biological functions. Methods The expression vector containing vWF-A1 domain was constructed by genetic engineering, and the recombinant vWF-A1 was expressed in E.coli. Then Balb/c mice were immunized by purified rvWF-A1. After fusion of immunized mouse spleen cells with myeloma SP2/0 cells, the hybridoma cells were screened with HAT culture medium. ELISA was used to select the positive... |
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| Keywords: | von Willebrand factor A1 domain prokaryotic expression platelet monoclonal antibody |
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