Relationship between nm23H1 genetic instability and clinical pathological characteristics in Chinese digestive system cancer patients |
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Authors: | Yang Yue-Qin Wu Liang Chen Jin-Xing Sun Jian-Zhong Li Meng Li Dong-Mei Lu Hai-Ying Su Zhi-Hong Lin Xin-Qiu Li Ji-Cheng |
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Institution: | 1. Institute of Cell Biology,Zhejiang University,Hangzhou 310058,Zhejiang Province,China 2. Zhejiang Qingtian People's Hospital,Qingtian 323900,Zhejiang Province,China 3. Zhejiang Tonglu People's Hospital,Tonglu 311500,Zhejiang Province,China |
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Abstract: | AIM: To study the relationship between nm23H1 gene genetic instability and its clinical pathological characteristics in Chinese digestive system cancer patients. METHODS: Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used to analyze the microsatellite instability (MSI) and loss of heterozygosiLy (LOH). Immunohistochemistry was employed to detect the expression of nm23H1. RESULTS: The MSI was higher in TNM stage I II than in stage ITI 1V of gastric, colonic and gallbladder carcinomas. The LOH was higher in TNM stage III IV than in stage I II of gastric, colonic and hepatocellular carcinomas. Lymphatic metastasis was also observed. The expression of nm23H1 protein was lower in TNM stage III IV than in stage I II of these tumors and in patients with lymphatic metastasis.The nm23H1 protein expression was higher in the LOH negative group than in the LOH positive group. CONCLUSION: MSI and LOH may independently control the biological behaviors of digestive system cancers. MSI could serve as an early biological marker of digestive system cancers. Enhanced expression of nm23Ht protein could efficiently inhibit cancer metastasis and improve its prognosis. LOH mostly appears in late digestive system cancer. |
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Keywords: | nm23H1 Gastric cancer Colonic cancer Hepatocellular carcinoma Gallbladder carcinoma |
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