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| 高效液相色谱法测定阿替洛尔片的含量 | |
| 引用本文： | 刘红莉,赫晓军,曹凤习,刘俊红,马钱钱. 高效液相色谱法测定阿替洛尔片的含量[J]. 中国药业, 2009, 18(15): 23-24 |
| 作者姓名： | 刘红莉 赫晓军 曹凤习 刘俊红 马钱钱 |
| 作者单位： | 1. 河北省药品检验所,河北,石家庄,520001 2. 河北维尔康制药有限公司,河北,石家庄,050031 3. 山西长治医学院,山西,长治,046000 |
| 摘 要： | 目的建立测定阿替洛尔及其片剂中阿替洛尔含量的高效液相色谱（HPLC）。方法采用外标法。色谱柱为Inertsil ODS-3柱（250mm×4．6mm，5μm），以磷酸盐缓冲液（取磷酸二氢钾6．8g，加水溶解并稀释至1000mL，用磷酸调pH至3．0）700mL加甲醇300mL与辛烷磺酸钠1．30g混匀，作为流动相，检测波长为226nm，流速为1．0mL／min。结果阿替洛尔质量浓度在10-300μg／mL范围内与峰面积线性关系良好，回归方程为A=43．1726C-116．3178（r=0．9996）。结论HPLC法简单、准确，可用于阿替洛尔片的含量测定。 |
| 关 键 词： | 高效液相色谱法 阿替洛尔片 含量测定 |
Content Determination of Atenolol Tablets by HPLC |
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| Liu Hongli,He Xiaojun,Cao Fengxi,Liu Junhong,Ma Qianqian. Content Determination of Atenolol Tablets by HPLC[J]. China Pharmaceuticals, 2009, 18(15): 23-24 | |
| Authors: | Liu Hongli He Xiaojun Cao Fengxi Liu Junhong Ma Qianqian |
| Affiliation: | 1. Hebei Irtsititute for Drug Control, Shijiazhuang, Hebei, China 520001; 2. Hebei Welcome Pharmaceutical Co., Ltd., Shijiazhuang, Hebei, China 050031; 3. Changzhi Medical College, Changzhi, Shanxi, China 046000) |
| Abstract: | Objective To determine the contents of atenolol in atenolol and Atenolol Tablets. Methods HPLC was carried using the Inertsil ODS- 3（250 mm×4. 6 mm, 5 μm） column. The mixture of 700 mL phosphate buffer solution （dissolving 6. 8 g of potassium dihydrogen phosphate with water and diluting to 1 000 mL, adjusting pH to 3.0 with phosphoric acid）, 300 mL methanol and 1.30 g sodium octanesulfonate as the mobile phase. The detection wavelength was 226 nm. The flow rate was 1.0 mL/min. Results Atenolol showed the good linearity in the range of 10-300 μg/mL, the regression equation was： A =43. 172 6C-116. 317 8 （r=0. 999 6）. Conclusion The method is simple and accurate. It is suitable to determine the content of Atenolol Tablets. |
| Keywords: | HPLC Atenolol Tablets content determination |
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