贝那普利联合曲尼司特对糖尿病肾脏疾病大鼠尿蛋白排泄率的影响

目的 探讨贝那普利联合曲尼司特对糖尿病肾脏疾病（diabetic kidney disease,DKD）大鼠尿蛋白排泄率的影响.方法 选择雄性SD大鼠40只,采用随机数字表法随机分为正常对照组10只,手术组30只,手术组应用单侧肾切除加高糖高脂饮食加小剂量链脲佐菌素（streptozotocin,STZ;40 mg/kg）方法制备糖尿病肾脏疾病大鼠模型,模型建立成功后再采用随机数字表法分为糖尿病肾脏疾病模型组（DKD组）、贝那普利用药组（B组）、贝那普利＋曲尼司特联合用药组（BQ组）,每组10只.B组在模型建立成功后予贝那普利10 mg·kg-1·d-1灌胃,BQ组在模型建立成功后予以曲尼司特（400 mg·kg-1·d-1）＋贝那普利（10 mg·kg-1·d-1）灌胃.于用药治疗开始后第12周末观察各组大鼠尿蛋白排泄率（urinary albumin excretion rate,UAER）、血肌酐（SCr）、尿素氮（BUN）及肾脏病理的变化.结果 与正常对照组相比,DKD组UAER明显增高（P＜0.05）,肾小球硬化程度较重（P＜0.05）;与B组相比,BQ组UAER更显著降低（P＜0.05）,肾小球硬化程度也显著降低（P＜0.05）.结论 贝那普利联合曲尼司特对DKD大鼠有明显的保护作用,其作用机制可能与降低UAER相关,并在一定程度上抑制肾间质纤维化.

Effects of benazepril combined with tranalist on urine albumin excretion in rats with diabetic kidney disease

Objective To investigate the effects of benazepril combined with tranalist on urine albumin excretion in rats with diabetic nephropathy. Methods Forty male SD rats were randomly divided into normal control group （N group, 10 rats） .and operaton group （30 rats） by using the method of random number table. A rat model of type 2 diabetic nephropathy （T2DN） was developed successfully by high glucose and high fat diet and low dose of streptozotocin （40 mg/kg） induced after unilateral nephrectomy in operation group. Operation group was randomly divided into model subgroup （T2DN subgroup）, benazepril treated subgroup （B subgroup）, combined benazepril with tranalist treatment subgroup （BQ subgroup） by using the method of random number table after the model was established successfully. Each subgroup had 10 rats. After the model was established successfully, B subgroup was given benazepril （10 mg · kg-1 · d - 1） by intragastric administration. BQ subgroup was given benazepril （to mg·kg· d-1） and tranalist （400 mg·kg-1·d-1） by intragastric administration. Urine albumin excretion, nitrogen （BUN） .serum creatinine （SCd and renal pathological changes were observed in each group at 12th week after treatment. Results As compared with N group, UAER was significantly increased （P〈0. 05）, and glomerular sclerosis was aggravated in T2DN subgroup （P〈 0.05）. As compared with B subgroup, UAER was decreased significantly （P〈0.05）, and glomerular sclerosis degree was significantly alleviated in BQ subgroup （P〈0. 05）. Conclusious Application of benazepril combined with tranilast has obvious protective effect on DN rats, which may be related to the decrease of urinary protein excretion and inhibition of renal interstitial fibrosis related to a certain extent.