类风湿关节炎患者血清白介素-33、白介素-35表达及其临床意义

目的:初步探讨白介素-33(IL-33)、白介素-35(IL-35)在类风湿关节炎(RA)发病中的意义。方法:酶联免疫吸附法(ELISA)检测50例RA患者(其中RA活动组25例,RA缓解组25例)、25名健康对照组血清IL-33、IL-35水平,并与临床资料进行相关性分析。结果:RA活动组患者血清IL-33水平(332.02±31.43)pg/ml显著高于RA缓解组(227.56±48.02)pg/ml及健康对照组(77.03±20.68)pg/ml,差异有统计学意义(P<0.05),RA缓解组与健康对照组差异有统计学意义(P<0.05);RA活动组血清IL-35水平(236.14±32.20)ng/L显著低于RA缓解组(353.21±24.61)ng/L及健康对照组(349.60±28.45)ng/L,差异有统计学意义(P<0.05),RA缓解组与健康对照组差异无统计学意义(P>0.05)。RA患者血清IL-33与IL-35水平之间呈显著负相关(r=-0.473,P<0.05);血清IL-33水平与关节肿胀数、关节压痛数、血沉(ESR)、C反应蛋白(CRP)、DAS28评分、抗CCP抗体呈正相关(P<0.05);血清IL-35水平与关节肿胀数、关节压痛数、ESR、CRP、DAS28评分、类风湿因子(RF)、抗CCP抗体呈负相关(P<0.05)。结论:RA活动组患者血清IL-33水平升高、IL-35水平减低,二者可能参与了RA发生发展,并可作为RA病情活动的预测指标。

Expression and clinical significance of interleukin-33 and interleukin-35 in the serum of patients with rheumatoid arthritis

Objective:To investigate the expression and its clinical significance of serum interleukin-33(IL-33) and interleukin-35(IL-35) in patients with rheumatoid arthritis(RA).Methods:Enzyme-linked immunosorbent assay(ELISA) was used to detect the blood serum levels of IL-33 and IL-35 in 50 RA patients(in whom 25 were in active stage and another 25 were in remission) and 25 healthy subjects.The results were analyzed and compared with the patients′ clinical data.Results: The serum IL-33 level was higher in patients in active stage(332.02±31.43) pg/ml compared with those in remission(227.56±48.02)pg/ml and the controls(77.03±20.68)pg/ml,which suggested statistical difference(P<0.05),whereas the level of IL-35 was lower for the former(236.14 ±32.20)ng/L as compared with the latter two(353.21±24.61 and 349.60±28.45)ng/L,respectively.Although the results were statistically different(P<0.05),yet,there was no significant difference between patients in remission stage and controls(P>0.05).Generally,the serum level of IL-33 in RA patients was negatively correlated with IL-35(r=-0.473,P<0.05).The IL-33 level was positively correlated with swollen joint count,tender joint count,erythrocyte sedimentation rate(ESR),C-reactive protein(CRP) DAS28 scores and anti-CCP(P<0.05),but IL-35 level was in negative correlation with those indicators(P<0.05) Conclusion: Elevated IL-33 but decreased IL-35 levels were seen in RA patients in active disease stage,suggesting that the two factors may be involved in the pathogenesis of RA and serve as potential indicators for estimation of this disease.