白桦脂酸对质粒急性感染模型小鼠的HBV DNA抑制作用研究

桦木酸是一种天然存在的五环三萜系化合物，它具有抗逆转录病毒，抗疟疾和抗炎特性。该研究主要目的是观察白桦脂酸对Payw1.3质粒急性感染小鼠模型的HBV DNA复制的抑制作用。实验的Payw1.3质粒急性感染小鼠模型（n=15）采用水动力转染技术构建，随机分为PBS对照组（n=5），白桦脂酸药物治疗组（n=5）及拉米夫定对照组（n=5），建模成功后次日分别予以白桦脂酸（100 mg·kg^-1），拉米夫定（50 mg·kg^-1），PBS药物灌胃，每日1次，连续7 d，分别在3，5，7 d眼眶静脉后采血，ELISA法测血清中HBsAg及HBeAg表达，并在7 d后处死小鼠，southern法检测肝组织HBV DNA表达。结果发现与PBS对照组相比，5 d时白桦脂酸能明显抑制Payw1.3质粒急性感染小鼠HBsAg表达（P<0.05），与拉米夫定效果无明显差异。与对照组相比，白桦脂酸和拉米夫定对HBeAg表达均无明显抑制作用，白桦脂酸和拉米夫定对肝组织HBV DNA表达均有抑制作用。这些结果揭示了白桦脂酸能够抑制Payw1.3质粒急性感染小鼠血清HBsAg表达及肝脏内HBV DNA的复制。

Research on HBV DNA inhibition of plasmid acute infection mouse with betulinic acid

Betulinic acid is a naturally occurring pentacyclic triterpenoid, which has antiretroviral, antimalarial, and anti-inflammatory properties. The purpose of this study is to investigate the HBV DNA replication inhibition in the mouse model with betulinic acid. Hydrodynamic injection method via the tail vein with the Payw1.3 plasmid was used to establish the animal mode(n=15), and the mice were randomly divided into the PBS control group(n=5), Betulinic acid treatment group(n=5) and lamivudine control group(n=5). The day after successful modeling, the mice would have taken Betulinic acid (100 mg·kg^-1), lamivudine (50 mg·kg^-1), PBS drugs orally, once daily for 7 days, blood samples were acquired from the orbital venous blood at 3,5,7 days after the administering, HBsAg and HBeAg in serum concentration were measured by ELISA and the mice were sacrificed after 7 days, HBV DNA southern detections were used with part of mice livers.The results showed that betulinic acid significantly inhibited the expression of HbsAg in the mice model at the fifth day compared with the control group, and there was no significant differences between the effects of lamivudine and the PBS control group; both the betulinic acid and lamivudine groups had no significant inhibition for the HBeAg expression; the HBV DNA expressions of the liver tissue from the betulinic acid and lamivudine groups were inhibited compared with the control group. Taken together, these results reveal betulinic acid can inhibit the HBsAg expression and replication of the liver HBV DNA in the mouse model.