骨髓间充质干细胞与K562细胞共培养对IL-8表达的影响

为了探讨骨髓间充质干细胞（mesenchymal stem cells,MSCs）与白血病细胞共培养后对血管生成因子IL-8表达的影响,本研究建立了MSC和K562细胞的共培养体系,包括直接接触和MSC条件培养液,应用RT-PCR方法检测MSC和K562细胞IL-8mRNA的表达。结果显示,与MSC直接接触和经MSC条件培养液作用后,K562细胞IL-8的表达均明显高于K562细胞单独培养组（p〈0.01）。与K562细胞共培养的MSC,其IL-8的表达亦明显高于单独培养的MSC（p〈0.01）。结论：MSC与K562细胞通过直接接触和某些细胞因子相互作用可促进血管生成因子IL-8的表达。

Effect of Co-culture of Bone Marrow Mesenchymal Stem Cells and K562 Cells on Expression of Interleukin 8

To investigate the effects of interaction between human bone marrow mesenchymal stem cells (MSCs) and K562 cells on the expression of proangiogenic factor IL-8, the K562 cells were co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium while the controlled K562 cells were cultured alone. The IL-8 gene expression of K562 cells and MSCs was determined by RT-PCR. The results indicated that the expression of IL-8 in K562 cells when co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium was obviously higher than that of K562 cells cultured alone (p<0.01). MSCs co-cultured with K562 cells also had an increased level of IL-8 compared with MSCs cultured alone (p<0.01). It is concluded that the interaction between MSCs and K562 cells via direct contact and the cytokine network promotes expression of IL-8.