表皮生长因子受体抑制剂AG1487对舌鳞癌细胞Tca8113的抑制增殖作用

目的:了解表皮生长因子受体抑制剂AG1487对舌鳞癌细胞的抑制增殖作用。方法:应用不同浓度(0、50、100、200nmol/L)的AG1487处理培养的舌鳞癌细胞Tca8113,采用MTT方法和流式细胞技术测定AG1487对细胞生长曲线和细胞周期分布的影响。结果:经不同浓度AG1487处理的肿瘤细胞,随抑制剂浓度的增加,其生长曲线逐渐向下移动,即随浓度的增加,抑制细胞增殖的作用逐渐增强。同时,AG1487使细胞周期的分布发生改变,G1期细胞显著增加,而S期细胞则明显减少;AG1487对细胞周期的影响具有明显的时间和剂量依赖关系。结论:表皮生长因子受体抑制剂AG1487可对舌鳞癌细胞产生明显的增殖抑制作用。

Effects of epidermal growth factor receptor inhibitor AG1487 on the proliferation of human tongue cancer Tca8113 cells

Objective:To investigate the inhibitory effect of epidermal growth factor receptor inhibitor AG1487 on the proliferation of tongue cancer cells. Methods:Tongue squamous cell carcinoma Tca8113 cells were treated by epidermal growth factor receptor inhibitor Tyrphostin AG1487 at 50,100 and 200 nmol/L respectively for 10 days.MTT method and flow cytometry were employed to investigate the inhibitory effects of AG1487 on the proliferation of Tca8113 cells.Results:AG1487 at 50~200 nmol/L inhibited the proliferation of Tca8113 cells in a dose-and-time dependant manner in 10-day-culture,increased G_1 phase cell population and decreased S phase cell population in 3-day-culture.Conclusion:Epidermal growth factor receptor inhibitor AG1487 may retard cell cycle, and then inhibit the proliferation of tumor cells.