膀胱移行细胞癌中survivin基因和血管内皮生长因子的表达和意义

目的：探讨survivin基因和血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）在膀胱移行细胞癌中的表达和意义。方法：用免疫组化二步法检测58例膀胱移行细胞癌组织及20例正常膀胱粘膜组织中VEGF和survivin的表达。结果：20例正常膀胱粘膜组织中VEGF、survivin表达均为阴性；survivin、VEGF在膀胱移行细胞癌阳性表达分别为33例（56．9％）、30例（51．7％）。肿瘤不同分级中随恶性程度的增高，表达增高，Ⅰ级与Ⅲ级比较，差异有显著性意义（P〈0．05）。不同临床分期中随分期的增高，表达增高，Tis—T1与T2-T4比较，差异有显著性意义（P〈0．05）。survivin、VEGF在膀胱移行细胞癌表达呈正相关（r=0．385，P〈0．01）。结论：VEGF和survivin在膀胱移行细胞癌的发生、发展过程中起着重要的作用。survivin基因可能参与了肿瘤血管的形成。

Expression and clinical significance of VEGF and survivin in bladder transitional cellcarcinoma

Objective：To investigate the expression and clinical significance of VEGF and survivin in bladder transitional cell carcinoma（TCC）. Methods：The expression of VEGF and survivin was studied by Envision immunohistochemisty in 20 cases of normal urinary bladder tissues and 58 cases of bladder TCC. Results： All the normal urinary bladder tissues showed negative staining pattern of VEGF and survivin. The positive rates of VEGF and survivin in 58 cases of bladder TCC were 51.7% and 56.9%, respectively. The expression of VEGF and survivin increased with grade, the difference of positive rate between Grade Ⅰ and Grade Ⅲ was significant（ P 〈 0.05 ）, the difference of positive rate between Tis - T1 and T2 - T4 was significant （ P 〈 0.05 ）. The expressions of VEGF and survivin were positively correlated in bladder TCC（ r = 0.385 ,P 〈0.01 ）. Conclusion： The abnormal expression of VEGF and survivin play an important role in the occurrence and progress of the bladder TCC. Survivin may be related to generation of vessels in bladder carcinoma.