Cytogenetic and molecular analysis of the satellite DNA of the genusCtenomys (Rodentia Octodontidae) from Uruguay |
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Authors: | A. Novello M. N. Cortinas M. Suárez H. Musto |
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Affiliation: | (1) Sección Genética Evolutiva, Facultad de Ciencias, T. Narvaja 1674, 11200 Montevideo, Uruguay;(2) Sección Bioqumica, Facultad de Ciencias, Montevideo, Uruguay |
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Abstract: | The genus of subterranean rodentsCtenomys presents the widest range of variability in diploid number among mammals (from2n=10 to2n=70). In Uruguay, this variability is observed in karyotypes with2n=44, 50 or 58 and two geographically isolated populations with 70 chromosomes but different karyotypic structure. The last three populations were analyzed in the present study. They present a satellite DNA, which was isolated from genomic DNA afterAlul digestion.In situ hybridization showed that this satellite DNA is located in the centromeric region of a few chromosomes, coincident with Hoechst 33258 staining and C-banding patterns. A similar satellite DNA was detected in Argentinian species of this genus. We established that, in spite of differences in number of positive heterochromatic blocks per karyotype, the C value is the same in the three populations studied. The nature and possible evolutionary path of this repeated DNA is discussed.Accepted for publication by M. Schmid |
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Keywords: | Ctenomys evolution heterochromatin rodents satellite DNA |
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