牛膝多糖对老龄大鼠非特异性免疫功能的影响

目的: 探讨牛膝多糖(ABPS)对老龄大鼠非特异性免疫功能的影响。 方法: 牛膝多糖(ABPS) 在老龄大鼠背部皮下注射21 d，镜检计外周血白细胞数（WBC），HiCN法测定血红蛋白（Hb）含量，用全自动生化分析仪测定主要生化指标，用含10 ％小牛血清的RPMI-1640细胞培养液贴壁培养肺泡巨噬细胞（AMΦ）和腹腔巨噬细胞（PMΦ）2 h，并用全自动生化分析仪测定其乳酸脱氢酶（LDH）和酸性磷酸酶(ACP)活性，摄取中性红试验检测巨噬细胞(MΦ)的吞噬功能，比色法检测脾脏、肝脏、大脑、肾脏和血清中的脂质过氧化物(LPO)和还原型谷胱甘肽(GSH)含量。结果: 牛膝多糖(ABPS)试验组老龄大鼠AMΦ和PMΦ内ACP、LDH的活性均高于对照组 （均P＜0.01），AMΦ和PMΦ摄取中性红的能力也高于对照组（均P＜0.01）； 且大脑、肝脏、脾脏中的LPO含量低于对照组（P＜0.05,P＜0.01），GSH含量高于对照组（P＜0.05）。 结论: 牛膝多糖(ABPS)具有激活老龄大鼠AMΦ和PMΦ作用，能增强机体非特异性免疫功能。

Effects of achyranthes bidentata polysaccharides on non-specific immune function of old rats

AIM: To study the effect of achyranthes bidentata polysaccharide (ABPS) on non-specific immune function of old rats. METHODS: After subcutaneous injection of ABPS (50 mg/kg body weight) into the back of the old rats for 21 days, the WBC and the levels of hemoglobin(Hb) and chief biochemical index in blood of old rats were determined. After the alveolar macrophages (AMΦ) and peritoneal macrophages (PMΦ) cultured with 10% fetal bovine serum-RPMI-1640 for 2 h in vitro, the activities of lactate dehydrogenase(LDH) and acid phosphatase(ACP) in the AMΦ and PMΦ were detected by automatic biochemistry analyzer, and the abilities in devouring neutral red in the AMΦ and PMΦ were observed. The levels of lipid peroxide(LPO) and reduced glutathione(GSH) in some immune organs like spleen, liver, brain, kidney and in serum were also detected.RESULTS: The levels of ACP and LDH in the AMΦ or PMΦ were increased (P<0.01), the ability of AMΦ or PMΦ in devouring neutral red was increased (P<0.01), the levels of LPO were diminished and the contents of GSH raised in the liver, brain and spleen (P<0.05 or P<0.01). CONCLUSION: ABPS has the functions of activating AMΦ and PMΦ， and enhances non-specific immunity in old rats.