瑞芬太尼预先给药对大鼠心肌缺血再灌注时心肌细胞凋亡相关基因和炎性相关基因表达的影响

目的 探讨瑞芬太尼预先给药对大鼠心肌缺血再灌注时心肌细胞凋亡相关基因及炎性相关基因表达的影响.方法 健康成年雄性SD大鼠24只,体重280～320 g,采用结扎左冠状动脉前降支30 min,再灌注45 min的方法 建立心肌缺血再灌注模型,随机分为3组(n=8):假手术组(S组)动脉下仅穿线,不结扎;缺血再灌注组(IR组)行心肌缺血再灌注;瑞芬太尼预先给药组(R组)以6 μg·kg-1·min-1的速率经股静脉输注瑞芬太尼,15 min后行心肌缺血再灌注.于再灌注45 min时处死大鼠,取左心室心尖部缺血区心肌组织,应用信号转导基因芯片技术检测心肌细胞凋亡相关基因及炎性相关基因的表达.结果 与S组比较,IR组Bax基因,Bcl-2基因、Bcl-2L1基因和Birclb基因表达下调;肿瘤坏死因子α(TNF-α)基因、细胞间粘附分子1(ICAM-1)基因表达上调,Birc3基因、白细胞介素2(IL-2)基因、IL-2rα基因、IL-4基因表达正常.与IR组比较,R组Bar基因、Bcl-2基因、Bcl-2L1基因Birclb和Bitc3基因表达上调,ICAM-1基因表达下调,IL-2基因、IL-2rα基因、IL-4基因、TNF-α基因表达正常.与S组和R组比较,IR组Bcl-2/Bax基因比降低(P<0.05).结论 瑞芬太尼预先给药可减轻大鼠心肌缺血再灌注损伤,其机制与上调抗心肌细胞凋亡相关基因表达和下调促炎性相关基因表达有关.

Effects of remifentanil pretreatment on expression of cardiomyocyte apoptosis- and inflammation-related genes in rats with myocardial ischemia-reperfusion injury

Objective To investigate the effects of remifentanil pretreatment on the expression of cardiomyocyte apoptosis- and inflammation-related genes in rats with myocardial ischemia-repeffusion (IR) injury.Methods Twenty-four adult male SD rats, weighing 280-320 g, were randomly divided into 3 groups (n = 8 each): sham operation group (group S), group IR, and remifentanil pretreatment group (group R). Myocardial IR was produced by occlusion of left anterior descending branch (LAD) of coronary artery for 30 min followed by reperfusion and myocardial tissues in ischemie area were obtained for determination of the expression of cardiomyocyte apeptosis- and inflammation-related genes. Results Compared with group S, the expression of Bax,Bcl-2, Bcl-2L1 and Birclb genes was down-regulated, expression of TNF-α and ICAM-1 genes up-regulated and expression of Bire3, IL-2, IL-2rα and IL-4. genes normal in group IR. Compared with group IR, the expression of Bax, Bcl-2, Bcl-2L1, Birclb and Birc3 genes was up-regulated, expression of ICAM-I gene down-regulated and expression of IL-2, IL-2rα, IL-4 and TNF-a genes normal in group R. Bcl-2/Bax gene ratio was significantly lower in group IR than in group S and R (P < 0.05). Conclusion Remifentanil pretreatment can attenuate myocardial IR injury by up-regulating the expression of anfi-cardiomyocyte apoptosis-related genes and down-regulating the expression of proinflammation-related genes in rats.