女性宫颈高危型HPV感染状况及其与宫颈病变剂量—效应关系分析

目的 探讨女性宫颈高危型人乳头瘤病毒(hrHPV)感染现状并分析hrHPV病毒载量与宫颈病变之间剂量—效应关系。方法 对26294份宫颈细胞学送检标本采用杂交捕获第二代方法(HC-Ⅱ)检测HPV-DNA含量，病毒载量以相对光单位(RLU)与界值点(Cutoff)比值衡量。以RLU/CO≥1.0为阳性。宫颈病变按组织学活检以CIN2及以上级别病变为病例。假设检验采用χ²检验；用Mantel趋势分析分析剂量—效应关系。结果 26294例受检对象总体阳性检出率为26.74%，年龄分布在15~84岁之间，平均年龄为（33.54±8.43）岁。hrHPV病毒载量与宫颈病变存在剂量—效应关系(χ_trend=8.000，P<0.001)。结论 广东省女性hrHPV感染阳性检出率较高；hrHPV病毒载量与宫颈病变之间存在剂量—效应关系。

Analysis of High-risk HPV Cervical Infection and Its Dose-effect Relationship between hrHPV Virus Load and Cervical Lesion

Objective To analyze high risk human papillomavirus (hrHPV) infection in female cervix and determine the dose-effect relationship between hrHPV virus load and cervical lesion. Methods HPV-DNA virus load of 26294 cervical samples were detected by hybrid captureII(HC-II) system. The viral load was assessed by ratios of relative light units per cut off value (RLU/CO). Samples with relative light unit per cutoff value (RLU/CO) of ≥1.0 was defined as positive. Cervical lesions were detected by biopsies, cervical intraepithelial neoplasia geade 2 (CIN2) or higher grade cervical lesions were determined as abnormal case. χ² test was used for the statistical test and Mantel trend analysis was used to analyze the dose effect relationship between hrHPV virus load and cervical lesion.Results The positive rate of hrHPV infection in 26294 samples was 26.74%, the age distribution ranged from 15 to 84 year old, the average age are of (33.54±8.43) years. Our statistical results showed dose effect relationship between hrHPV virus load and cervical lesion (χ_trend=8.000, P<0.001). Conclusion The positive detection rate of hrHPV in Guangdong province female was relatively high; there are dose effect relationship between hrHPV virus load and cervical lesion.