男性不育精浆弓形虫IgG与NO关系研究

目的　观察男性不育病人精浆弓形虫IgG与NO水平及其关系。 方法　采用ELISA方法测定 16 9例男性不育患者及 35例正常生育男性精浆弓形虫IgG抗体 (ATAb)和抗精子抗体 (AsAb) ,同时采用硝酸还原酶法测定精浆中NO水平。结果　男性不育病人精浆ATAb阳性率 18 35 % ,显著高于正常生育组的 2 86 % (P <0 0 5 ) ;男性不育组精浆AsAb阳性率为2 6 6 3% ,也显著高于正常生育组 (P <0 0 1)。男性不育组精浆中NO水平 14 6 6 8± 38 87μmol/L ,显著高于正常生育组 84 92± 2 6 72 μmol/L(P <0 0 1)。精浆弓形虫IgG抗体阳性的男性不育病人精浆NO水平 15 3 2 1± 4 1 6 0 μmol/L ,显著高于精浆ATAb阴性组的男性不育病人精浆的NO水平 135 81± 31 4 5 μmol/L(P <0 0 1)。精浆AsAb阳性的男性不育病人NO水平15 8 76± 4 3 0 0 μmol/L ,显著高于精浆AsAb阴性组的男性不育病人精浆的 133 82± 32 10 μmol/L(P <0 0 1)。精浆ATAb阳性的男性不育病人精浆AsAb的阳性率为 38 71% ,也显著高于精浆ATAb阴性组的男性不育病人精浆AsAb的阳性率2 3 91% (P <0 0 1)。结论　男性不育病人精浆ATAb与精浆NO水平具有相关性 ,同时也与抗精子抗体的产生可能有着一定的关系。

Relations between anti-Toxoplasma IgG antibody in seminal plasma and NO level in infertile patients

Aim To observe the variations of anti-Tg IgG antibody(ATAb)and anti-sperm antibody(AsAb)in seminal plasma infertile patients and to study its relation between NO level.Methods The ELISA method was used to determine the seminal plasma's anti-Tg IgG antibody and AsAb respectively.The samples were collected from 169 patients with infertility and 35 males with normal fertility.The NO levels in their semina were determined by means of nitric acid reducase.Results the ATAb positivity rate in the masculine infertility patients was 18.25%.which was remarkably higher than 2.86% in the normal fertility group(P<0.05).The NO level in the semina from the infertility group was 146.68±38.87μmol/L,which was obviously higher than 84.92±26.72μmol/L(P<0.01)in the fertility group.Among the infertility patients the NO level of anti-Tg IgG antibidy positive group was 153.21±41.60μmol/L,which was also strikingly higher than 135.81±31.45 μmol/L(P<0.01)of the ATAb negative group.Conclusion The ATAb in semina from the masculine infertility patients is possibly closely related to the high level of NO.