| 黄芪中黄芪总皂苷的大孔树脂纯化工艺研究 |
| |
| 引用本文： | 隋玉兰,李兆翌. 黄芪中黄芪总皂苷的大孔树脂纯化工艺研究[J]. 中国实用医药, 2010, 5(31): 1-2 |
| |
| 作者姓名： | 隋玉兰 李兆翌 |
| |
| 作者单位： | 1. 山东省济宁市第一人民医院,272000
2. 杭州,浙江中医药大学 |
| |
| 摘 要： | 目的优选出分离纯化黄芪中黄芪总皂苷的最佳工艺条件。方法采用动态吸附-解析法,用超高效液相色谱法测定黄芪甲苷的含量,优选出最佳纯化条件。结果 D101树脂对黄芪总皂苷的纯化最优,以2BV/h吸附速率吸附,5BV70%乙醇以3BV/h的流速进行洗脱效果最佳,黄芪甲苷的精制度为446.28%。结论 D101大孔吸附树脂可用于黄芪总皂苷的纯化。
|
| 关 键 词： | 黄芪 黄芪总皂苷 大孔树脂 |
Purification of total saponins of Astragalus in Radix Astragali |
| |
| SUI Yu-lan,LI Zhao-yi. Purification of total saponins of Astragalus in Radix Astragali[J]. China Practical Medical, 2010, 5(31): 1-2 |
| |
| Authors: | SUI Yu-lan LI Zhao-yi |
| |
| Affiliation: | .( People＇ s Hospital of Jining, Shandong Jining 272000, China) |
| |
| Abstract: | Objective To optimize the technological parameter of purification of the astragaloside IV. Methods With the extraction rate of the astragaloside IV, the optimized technological parameter was selected by UPLC. Results D101 was found to provide the best fit of separation and purification, take the best condition of 2BV/h current velocity,70% ethanol as the eluting reagent, and the eluting velocity was 3 BV/h. This method made the astragalosideIV have a refined degree of 446. 28%. Conclusion D101 resin could be used to refine the astragalosideiv in Radix Astragali. |
| |
| Keywords: | Radix Astragali Total saponins of Astragalus Macroporous resin |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
