微电极刺激大鼠杏仁核建立慢性癫痫动物模型

目的：建立杏仁核点燃的大鼠慢性癫痫模型。方法：在3．6％水合氯醛麻醉下，用漆包镍镙双极螺旋电极植入Wistar大鼠杏仁核，术后第7天，用微电极刺激大鼠杏仁核。记录脑电活动，同时观察大鼠行为变化。当脑电图上有发作后电位，即2～5Hz爆发性的尖波或棘慢波，波幅大于200μV，持续3s以上，同时按Racine分级法大鼠的行为表现达到4级或5级时可视为大鼠癫痫模型点燃成功。结果：34只Wistar大鼠被点燃。癫痫发作模式具有规律性、容易辨认、可重复诱发，癫痫发作程度可以量化的优点。6只为亚临床发作(即有典型的脑电图的棘波放电表现，但是行为不足4-5级)。结论：通过微电极刺激Wistar大鼠杏仁核可以建立稳定的大鼠癫痫慢性点燃模型。

The Establishment of Model of Amygdala Kindling Chronic Epilepsy with Wistar Rat

Objective: To establish the model of amygdala kindling chronic epilepsy with wistar rat. Methods: The amygdalas of 60 6 -8-weeks-old wistar rats were stimulated with constant pulsating current that had frequencies of 50 Hz, pulsating duration of 1. 0ms, train duration of 1 s and inter stimulus interval of 24 hours. Results: The results showed the 34 rats were kindled in 5 -20 day. The models were relatively stable. The paroxysm of epilepsy had a regular pattern, and was easily recognized and reproduced. The seizures could be quantified. Conclusion: To stimulate wistar rats at amygdalas with constant pulsating current may induces chronic kindling epilepsy and to establish relatively stable animal model.