Effects of Taohong Siwu Decoction Ⅱ in the Chick Chorioallantoic Membrane （CAM） Assay and on B16 Melanoma in Mice and Endothelial Cells ECV304 Proliferation

To investigate the anti-angiogenesis action of Taohong Siwu Decoction Ⅱ （THSWD Ⅱ）. Methods： The chick chorioallantoic membrane （CAM） assay was adopted to study the anti-angiogenesis action of THSWD Ⅱ; the MTT test was used to investigate its effect on proliferation of the human umbilical vein endothelial cells ECV304; and the immunohistochemical method was used to observe the effect of THSWD Ⅱ on the expression of kinase insert domain containing receptor/fetal liver kinase 1 （KDR/Flk-1） and the microvessel density （MVD） of B 16 melanoma in mice. Results： After treatment with THSWD Ⅱ, the blood vessel index of CAM and the absorbency of ECV304 in the THSWD Ⅱ 1mg/ml group and the 2mg/ml group decreased significantly （P〈0.01）; the weight, the expression of KDR/Flk-1 and the MVD of B16 melanoma in mice reduced significantly in the THSWD Ⅱ 5g/kg group, the 10g/kg group and the TSHSWD10g/kg plus cyclophosphamide group （P〈0.01）. Conclusion： THSWD Ⅱ has the actions of anti-angiogenesis, and inhibiting the proliferation of ECV304 cells and the growth of B16 melanoma. The clinical anti-tumour mechanism is considered to be related possibly to its anti-angiogenesis action by inhibiting the expression of KDR/FIK- 1.

Effects of taohong siwu decoction II in the chick chorioallantoic membrane (CAM) assay and on B16 melanoma in mice and endothelial cells ECV304 proliferation.

OBJECTIVE: To investigate the anti-angiogenesis action of Taohong Siwu Decoction II (THSWD II). METHODS: The chick chorioallantoic membrane (CAM) assay was adopted to study the anti-angiogenesis action of THSWD II; the MTT test was used to investigate its effect on proliferation of the human umbilical vein endothelial cells ECV304; and the immunohistochemical method was used to observe the effect of THSWD II on the expression of kinase insert domain containing receptor/fetal liver kinase 1 (KDR/Flk-1) and the microvessel density (MVD) of B16 melanoma in mice. RESULTS: After treatment with THSWD II, the blood vessel index of CAM and the absorbency of ECV304 in the THSWD II 1 mg/ml group and the 2 mg/ml group decreased significantly (P < 0.01); the weight, the expression of KDR/Flk-1 and the MVD of B16 melanoma in mice reduced significantly in the THSWD II 5 g/kg group, the 10 g/kg group and the TSHSWD 10g/kg plus cyclophosphamide group (P < 0.01). CONCLUSION: THSWD II has the actions of anti-angiogenesis, and inhibiting the proliferation of ECV304 cells and the growth of B16 melanoma. The clinical anti-tumour mechanism is considered to berelated possibly to its anti-angiogenesis action by inhibiting the expression of KDR/FIK-1.