肝动脉内化疗栓塞联合免疫治疗的实验研究

目的 探讨肝动脉化疗栓塞(TACE)联合免疫治疗的疗效.方法 采用新西兰大白兔VX2肿瘤株移植到肝脏的方法制作肝肿瘤模型30只;并随机平均分为生理盐水对照组(G1);常规TACE组(G2);TACE+肝动脉灌注细胞因子组(G3);TACE+脾动脉灌注细胞因子组(G4):TACE联合超液化碘油+细胞因子组(G5);每组6只.分别进行肝动脉插管治疗,观察各组的肿瘤组织内增殖细胞核抗原标记指数的表达;并用间接免疫荧光法检测兔外周血T淋巴细胞亚群的分布.结果 G3组、G4组、G5组PCNA LI显著小于对照组(G1)和G2组(P＜0.01);而G2组与G1组相比较,差异无显著性(P＞0.05);G5组与G3、G4组比较差异有显著性(P＜0.05).G2组外周血CD3+、CD4+淋巴细胞及CD4+/CD8+比值显著低于G1组(P＜0.05);而G3、G4组则高于G1组(P＜0.05);G5组则明显高于G2组(P＜0.01)和G3、G4组(P＜0.05).结论 TACE不能抑制肿瘤细胞的增殖活性;而TACE细胞因子却能明显抑制其增殖活性;TACE可使机体免疫功能在一定时间内下降,表现为外周血T淋巴细胞亚群分布的紊乱;而联合超液化碘油+细胞因子可改善免疫抑制状态.

Experimental research of hepatic arterial chemoembolization combined with immune therapy

Objective] To research the curative effect of hepatic arterial chemoembolization combined with immune therapy Method] Thirty New Zealand White rabbits bearing transplanted VX2 liver cancer were randomly grouped(6 rabbits each group) and treated by normal saline as control(G1),or TACE alone(G2),or TACE +injection of IL-2 and IFN-a via hepatic artery(G3),or TACE +injection of Cytokine via splenic artery(G4),or TACE combined with lipiodol emulsion+Cytokine(G5).The therapeutic effects were observed including ...