福辛普利对压力超负荷性心肌肥厚大鼠左心室心肌细胞bcl-2及bax基因表达的影响

目的：研究福辛普利（fosinopril）对压力超负荷性心肌肥厚大鼠bcl-2、bax基因表达的影响。方法：将36只Wistar大鼠随机分为A组（假手术组）、B组（腹主动脉缩窄组）、C组（腹主动脉缩窄组＋福辛普利给药组）。实验中以左心室重量指数（LVW/BW）作为判断心肌肥厚的指标;心肌细胞bcl-2、bax基因表达的变化用间接免疫荧光标记法比较。心肌细胞bcl-2、bax基因表达用流式细胞仪（Flow Cytometry FCM）测试。结果：B组与A组相比,心肌细胞bcl-2基因表达下降（P〈0.01）,bax基因表达增多（P〈0.01）;C组与B组相比,bcl-2基因表达增多（P〈0.01）,bax基因表达下降（P〈0.05）。结论：福辛普利可促进压力超负荷性心肌肥厚大鼠bcl-2基因的表达,抑制bax基因的表达,从而使心肌肥厚发生逆转。

THE EFFECTS OF FOSINOPRIL ON BCL-2 AND BAX GENES OF MYOCARDIAL HYPERTROPHY RAT WITH PRESSURE VERLOAD

Objective：To investgate the effects of fosinopril on the bcl-2 and bax genes of myocardial hypertrophy rat with pressure overload. Methods：36 Wistar rats were randomly divided into three groups,A group （ false operation group ） , B group （ abdominal aorta constriction group ） and C group（ abdominal aorta constriction ＋fosinopril treatment group） . The ratio of left ventricular weight vs body weight（LVW/BW）was regarded as the index of myocardial hypertrophy;Cardiomyocytes bcl-2, bax gene expression changes in comparison with the indirect immunofluorescence,which was teasted by Flow Cytometry （ FCM） . Result：Comparing with A group,B group is decreased obviously in the bcl-2 gene expression （P〈0. 01）,and increased in the bax gene expression （P〈0. 01）. Comparing with B group,C group is increased obviously in the bcl-2 gene expression （P〈0. 01）,and decreased in the bax gene expression （ P〈0 . 05 ） . Conclusion：Fosinopril could increase the expression of bcl-2 gene and decrease the expression of bax gene in the hypertrophy rats with pressure verload.