Nanocarriers for topical administration of resveratrol: A comparative study |
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Authors: | Immacolata Scognamiglio Daniela De Stefano Virginia Campani Laura Mayol Rosa Carnuccio Gabriella Fabbrocini Fabio Ayala Maria Immacolata La Rotonda Giuseppe De Rosa |
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Institution: | 1. Department of Pharmaceutical and Toxicological Chemistry, Faculty of Pharmacy, University of Naples Federico II, via Montesano 49, Naples, Italy;2. Department of Experimental Pharmacology, Faculty of Biotechnology, University of Naples Federico II, via Montesano 49, Naples, Italy;3. Department of Systematic Pathology, Division of Clinical Dermatology, University of Naples Federico II, via S. Pansini 5, Naples, Italy |
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Abstract: | The trans-resveratrol (t-res), a non-flavonoid polyphenol extracted from different plants, has recently earned interest for application on the skin for different applications. In this work, the potential of nanocarriers, namely transfersomes and ethanol-containing vesicles, to deliver t-res into/through the skin was investigated. Thus, transfersomes with different surfactants, namely polysorbate 80 (Tw80), sodium cholate (SC) and sodium deossicholate (SDC) and ethanol-containing vesicles with different lipid composition, namely soy phosphatidylcholine (SPC) and cholesterol (chol), encapsulating t-res were prepared and characterized. The nanocarriers had a mean diameter ranging between 83 and 116 nm with a high t-res encapsulation efficiency (≥70%). Moreover, cytotoxicity as well as the inhibition of production of reactive oxygen species (ROS) and lipid peroxidation, following incubation of H2O2-stimulated human keratinocyte (HaCaT) with t-res, as free or encapsulated into the nanocarriers, were investigated. Only blank nanocarriers containing Tw80 or ethanol were cytotoxic and led to increase of ROS, but this effect was not observed when using nanocarriers encapsulating t-res. Finally, permeation studies on porcine skin carried out on Franz diffusion cells, showed that only ethanol-containing vesicles based SPC were able to promote t-res permeation through the skin. |
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Keywords: | t-res trans-resveratrol Tw80 polysorbate 80 SC sodium cholate SDC sodium deossicholate SPC phosphatidylcholine chol cholesterol ROS reactive oxygen species HaCaT human keratinocyte MTT 3-(4 5-dimethylthiazol-2yl)-2 5-diphenyl-2H-tetrazolium bromide DMEM Dulbecco's modified Eagle's medium FBS fetal bovine serum HPLC high performance liquid chromatographic RP-HPLC reverse-phase chromatography H2DCFDA 2&prime 7&prime -dichlorofluorescin-diacetate |
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