| 重组人幽门螺杆菌尿素酶B亚单位的免疫学性质研究 |
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| 引用本文： | 鲁东水,毛旭虎,邹全明,吴超,张卫军. 重组人幽门螺杆菌尿素酶B亚单位的免疫学性质研究[J]. 第三军医大学学报, 2003, 25(10): 887-889 |
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| 作者姓名： | 鲁东水 毛旭虎 邹全明 吴超 张卫军 |
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| 作者单位： | 第三军医大学医学检验系临床微生物学与免疫学教研室,重庆,400038 |
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| 摘 要： | 目的 分析重组幽门螺杆菌尿素酶B亚单位的免疫学性质。方法 重组尿素酶B亚单位注射免疫BALB／c小鼠、家兔，双向琼脂扩散、ELISA、免疫印迹分析特异抗体的产生及性质。结果 重组尿素酶B亚单位能有效刺激机体的免疫应答，且产生的抗体能与天然幽门螺杆菌发生抗原抗体反应。结论 重组尿素酶B亚单位表现出天然幽门螺杆菌尿素酶B亚单位的免疫学性质，提示可作为幽门螺杆菌特异性抗体检测的包被抗原及幽门螺杆菌疫苗的有效成分。
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| 关 键 词： | 幽门螺杆菌 尿素酶B亚单位 基因重组 免疫学性质 |
| 文章编号： | 1000-5404(2003)10-0887-03 |
| 修稿时间： | 2002-06-28 |
Immunological characters of recombinant urease B subunit of Helicobacter pylori |
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| LU Dong shui,MAO Xu hu,ZOU Quan ming,WU Chao,ZHANG Wei jun. Immunological characters of recombinant urease B subunit of Helicobacter pylori[J]. Acta Academiae Medicinae Militaris Tertiae, 2003, 25(10): 887-889 |
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| Authors: | LU Dong shui MAO Xu hu ZOU Quan ming WU Chao ZHANG Wei jun |
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| Abstract: | Objective To determine the immunological characters of recombinant urease B subunit(rUreB) of H. pylori. Methods BALB/c mice and rabbits were immunized with rUreB. The specific antibodies to UreB were analyzed by agarose diffusion, ELISA and Western blot. Results The recombinant UreB could stimulate the immune response in vivo , and the specific antibodies could also react with UreB of H. pylori . Conclusion The recombinant UreB has the similar immunological characters with native UreB of H. pylori , suggesting that it could be used as the component of vaccine against H. pylori and as the coated antigen for the detection of the specific antibodies to UreB of H. pylori. |
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| Keywords: | H. pylori urease B subunit recombinant immunological character |
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