LGR5和E-cadherin在胃癌组织中的表达及临床意义

目的 探讨富含亮氨酸重复序列G蛋白偶联受体5（leucine-rich repeat-containing G protein-coupled receptor 5，LGR5）和 E-钙粘蛋白（E-cadherin）在胃癌组织中的表达及其临床意义。方法 收集2014年8月—2016年5月在中南大学湘雅医学院附属株洲医院手术切除的115例胃癌组织及相应20例癌旁正常组织标本。采用免疫组化SP法检测LGR5和E-cadherin的表达水平，分析LGR5与E-cadherin表达的相关性及其与胃癌患者临床病理特征和预后的关系。结果 LGR5和E-cadherin在胃癌组织中阳性表达率分别为62.6%和53.9%，与癌旁正常组织相比，差异有统计学意义（P<0.05）；LGR5和E-cadherin表达均与肿瘤大小、TNM分期、浸润深度、淋巴结转移及远处转移有关（P<0.05）；相关分析显示，LGR5与E-cadherin表达呈负相关（r=-0.318，P=0.001）。LGR5阴性表达患者的1年、3年总生存率高于阳性表达患者（P<0.001），E-cadherin阳性表达患者的1年、3年总生存率亦高于阴性表达患者（P<0.001）；多因素Cox回归分析显示，TNM分期Ⅲ~Ⅳ期、淋巴结转移、远处转移、LGR5表达阳性及E-cadherin表达阴性是影响胃癌患者预后的独立危险因素（P<0.05）。结论 LGR5在胃癌组织中高表达而E-cadherin表达缺失，可能导致胃癌的发生发展及不良预后。

Expression of LGR5 and E-cadherin in gastric cancer tissues and its clinical significance

Objective To investigate the expression of leucine-rich repeat-containing G protein-coupled receptor 5(LGR5)and E-cadherin in gastric cancer tissues and its clinical significance. Methods 115 gastric cancer tissues and 20 corresponding adjacent normal tissues were collected from surgical resection at the Affiliated Zhuzhou Hospital of XiangYa School of Medicine CSU between August 2014 and May2016. Immunohistochemical staining was performed to determine the expression of LGR5 and E-cadherin. The correlation between LGR5 and E-cadherin expression as well as its relationship with clinicopathological characteristics and prognosis of gastric cancer patients were analyzed. Results The positive rates of LGR5 and E-cadherin in gastric cancer tissues were 62.6% and 53.9%,respectively,compared with the adjacent normal tissues,the difference was statistically significant(P<0.05). The expression of LGR5 and E-cadherin in gastric cancer tissues were closely related to tumor size,TNM stage,depth of invasion,lymph node and distant metastasis(P<0.05). Spearman rank correlation analysis indicated that LGR5 expression was negatively correlated with E-c adherin expression in gastric cancer tissues(r =-0.318,P=0.001). The 1-year and 3-year overall survival rates of LGR5 negative patients were higher than that of positive patients(P<0.001). The 1-year and 3-year overall survival rates of E-cadherin positive patients were also higher than those of negative patients(P<0.001). Multivariable Cox regression analysis showed that TNM stage Ⅲ-Ⅳ,lymph node metastasis,distant metastasis,LGR5 positive expression,and E-cadherin negative expression were the independent factors for the prognosis of gastric cancer patients(P<0.05).Conclusion The high expression of LGR5 in gastric cancer tissues and the lack of E-cadherin expression may lead to the development and poor prognosis of gastric cancer.