人正常发育角膜上皮和角膜基质的凝集素化学研究

目的:研究正常人发育角膜上皮及基质中凝集素亲和糖蛋白的分布及变化,探讨糖类化合物对角膜发育的可能作用。方法:用五种生物素标记的凝集素:麦胚凝集素(Wheat Germ Agglutin WGA)、伴刀豆凝集素(Con-Canavlin A ConA)、花生凝集素(Peanut Agglutin PNA)、蓖麻凝集素(Ricinus Communis Agglutin I RCA-I)扁豆凝集素(Lens Culinaris A LCA)对人正常发育角膜进行凝集素组织化学研究。结果:1.WGA受体主要分布在角膜上皮细胞膜中,尤其在表层上皮细胞中有明显分布,在发育期间胚胎角膜的前部基质中也有WGA受体分布。2.ConA受体分布于角膜上皮细胞膜及细胞质内,在胚胎期角膜基质中有不均一分布。3.RCALCA受体主要分布在角膜上皮基底部细胞膜上。4.PNA受体在胚胎16周角膜上皮细胞中有较少量分布。结论:1.在人正常发育角膜组织中含有ConA、WGA、PNA、LCA、RCA亲和性糖蛋白,这些糖蛋白参与发育角膜构成。2.在人角膜发育成熟过程中,有凝集素亲和糖蛋白的分布变化,这种变化可能与角膜的发育成熟有关。眼科学报1996;12:178～182。

Lectin - binding Histochemical Study on Developing Corneal Epithelium and Stroma of Normal Human Being

Purpose:To study glycoconjugates in developing cornea of normal human Methods: Lectin - binding histochemical mathod with five biotin - coupled lectins (conA, LCA,PNA,WGA,RCA)Results: 1. WGA mainly stained the membrane of epithelial cells, espacially in surface cells. It also stained anterior stroma of embryo. 2. All the layers of epithelium could be stained by conA, uneven stain was seen in stroma of embryo corneas. 3. RCA,LCA receptor distributed in based of cornea epithalium. 4. PNA can only stain cornea epithalium of 16 wembryo. Conclusions: 1. Glycoconjugates of binding conA,WGA,PNA,LCA,RCA are in the developing comeas of human. 2. The distribution of these lectin recepters may chang with couneal developing. It may be related to the maturity of corneas. Eye Science 1996; 12 : 178 - 181.