基因p16及Cyclin D1在子宫内膜癌中的表达及意义

探讨抑癌基因Ｐ１６及细胞周期素Ｄ（ＣｙｃｌｉｎＤ１）在子宫内膜癌，交界性子宫内膜组织及正常子宫内膜组织中的表达规律及其与子宫内膜癌的关系。方法应用免疫组化ＳＡＢＣ法检测３８例子宫内膜癌，１９例子宫内膜增生过长及不典型增长，１０例正常子宫内膜组织中基因ｐ１６，ＣｙｃｌｉｎＤ１的表达。

Expression of p16 and Cyclin D1 in uterine endometrial carcinomas and its significance

AIM To study the expression and significance of p16 and Cyclin D 1 in human uterine endometrial carcinomas. METHODS The expression of p16 and Cyclin D 1 was exa mined in malignant, borderline and benign uterine endometrial tissues by immunohistochemistry SABC methods. Monoclonal antibodies against p16 and Cyclin D 1 were employed. RESULTS The positive rates of p16 expression among the malignant, borderline and benign tumors were 40%, 68% and 0 respectively. Significant difference was observed ( P <0.05) in malignant tumors. The expression of p16 was associated with pathological grades and no association was found with clinical phases. Cyclin D 1 expression was negative in benign tumors and the positive rates in malignant and borderline tumors were 53% and 21% respectively ( P <0.05). In uterine endometrial carcinomas, no association was found between clinical phases and pathological grades. CONCLUSION There is an overexpression of Cyclin D 1 and a lower expression of p16 in uterine endometrial carcinomas and the synergy of the expression of p16 and Cyclin D 1 may contribute to the development of uterine endometrial carcinomas.