姜黄素对食饵性高脂血症大鼠血浆脂蛋白代谢相关酶活性的影响

为了探讨姜黄素降血脂、抗动脉粥样硬化可能的酶学机理．用高脂膳食喂饲Wistar大鼠4周，造成食饵性高脂血症，然后用高、低两种剂量的姜黄素和阳性对照药血脂康、非诺贝特进行实验性治疗。给药3周后处死动物，比较治疗前后血清和肝脏总胆固醇及甘油三酯含量、血清高密度脂蛋白胆固醇和低密度脂蛋白胆固醇含量，同时测定血浆卵磷脂胆固醇酰基转移酶、肝素化血浆总脂解酶、脂蛋白脂酶和肝脂酶活性。结果发现，高、低剂量姜黄素、非诺贝特和血脂康均能使血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇含量降低，降甘油三酯作用高剂量姜黄素和非诺贝特最优，降总胆固醇和低密度脂蛋白胆固醇作用低剂量姜黄素和血脂康最优，高剂量姜黄素和非诺贝特能增加高密度脂蛋白胆固醇含量，同时能降低肝脏总胆固醇和甘油三酯含量。高、低剂量姜黄素能显著提高血浆卵磷脂胆固醇酰基转移酶活性．降低血浆游离胆固醇含量，高、低剂量姜黄素和非诺贝特能提高血浆总脂解酶和脂蛋白脂酶活性，高剂量姜黄素还能显著提高肝脂酶活性。结果提示，姜黄素具有明显的降低肝脏和血清脂质的作用，可能与提高血浆脂蛋白代谢相关酶的活性有关。

Effects of Curcumin on the Activities of Plasma Lipase and the Concentrations of Lipids in Hyperlipidemic Rats Induced by High Lipid Diet

Aim To investigate the possible mechanism of Curcumin's effect on decreasing plasma lipid and anti atherosclerosis. Methods Wistar rats were fed with high lipid diet 4 weeks to be hyperlipidemic model and then given experimental treatment with Curcumin, XUEZHIKANG and Fenofibrate as positive control drugs. After 3 week's treatment, the levels of total cholesterol (TC) and triglyceride (TG) in the serum and liver were determined. The level of serum high density lipoprotein cholesterol (HDLC), the level of plasma lecithin cholesterol acyltransferase (LCAT) activity , and the levels of postheparin total activity (PHTA), lipoprotein lipase (LPL) and hepatic lipase (HL) activities were all measured. Results The three drugs can decrease the serum TG level significantly, especially the high dosage of Curcumin and Fenofibrate. They can decrease the level of serum TC and low density lipoprotein cholesterol (LDLC), especially XUEZHIKANG and low dosage of Curcumin. The high dosage of Curcumin and Fenofibrate can increase the level of HDLC. Curcumin and Fenofibrate can decrease the level of TC in the liver. The high dosage of Curcumin and Fenofibrate can significantly decrease the level of TG in the liver. Curcumin can increase the LCAT activity in the plasma significantly and decrease the level of free cholesterol (FC)in the plasma. Curcumin and Fenofibrate can increase the level of PHTA. The high dosage of Curcumin can increase the level of HL activity. Curcumin and Fenofibrate can increase the level of LPL activity. Conclusion The results indicate that Curcumin has significant effect on down regulating serum and liver lipids. Meanwhile, it can up regulating the level of plasma lipase avtivities, which may be one of the possible mechanisms of Curcumin's effect on decreasing lipid and anti atherosclerosis.