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| pcDNA3转染HPV16E6基因对人宫颈癌细胞C33A细胞生长及周期的影响 | |
| 引用本文： | 杨秀坤,陈宣男,姜铮. pcDNA3转染HPV16E6基因对人宫颈癌细胞C33A细胞生长及周期的影响[J]. 第三军医大学学报, 2009, 31(9) |
| 作者姓名： | 杨秀坤 陈宣男 姜铮 |
| 作者单位： | 1. 吉林省通化市人民医院妇产科,吉林,通化,134001 2. 吉林大学公共卫生学院流行病与卫生统计学教研室,长春,130021 3. 中国人民解放军疾病预防控制所军队传染病控制中心,北京,100071 |
| 摘 要： | 目的 探讨HPV16E6对宫颈癌C33A细胞生长增殖的影响.方法 通过脂质体法将HPV16E6基因稳定转染宫颈癌C33A细胞,RT-PCR及Western blot分别检测转染细胞中HPV16E6 mRNA和蛋白表达水平,流式细胞仪检测HPV16E6基因转染对C33A细胞生长增殖与细胞周期的影响.结果 稳定转染获得了稳定表达pcDNA3-HPV16E6质粒的C33A细胞克隆(C33A-E6细胞,即pcDNA3/HPV16E6/C33A组)及稳定复制空载体质粒的细胞克隆(C33A-P细胞,即pcDNA3/C33A组),并将未转染的C33A细胞作为对照组.pcDNA3/HPV16E6/C33A组细胞的生长速度比pcDNA3/C33A组和对照组明显加快(P<0.05).在转染了HPV16E6基因的C33A细胞中,其DNA合成前期G1和静止期G0(G0/G1期)细胞百分率(39.3%)明显低于pcDNA3/C33A组(53.7%)和对照组(55.4%)(P<0.01);而处于S期细胞的百分率(38.3%)、DNA合成后期G2及分裂期M的细胞百分率(G2/M:32.9%),均明显高于pcDNA3/C33A组(S:29.4%;G2/M:17.0%)和对照组(S:28.0%;G2/M:16.6%)(P<0.01).pcDNA3/C33A组和对照组细胞周期的各期分布无明显差异(P>0.05).结论 HPV16E6可能通过某种途径参与了C33A细胞的周期调控,促进细胞分裂,从而促进C33A细胞的增殖. |
| 关 键 词： | 人乳头瘤病毒 官颈癌细胞 增殖 细胞周期 |
HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A |
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| YANG Xiu-kun,CHEN Xuan-nan,JIANG Zheng. HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(9) | |
| Authors: | YANG Xiu-kun CHEN Xuan-nan JIANG Zheng |
| Abstract: | Objective To investigate the transfection of human papilloma virus types 16E6 gene on the proliferation and apoptosis of cervical carcinoma cell line C33A. MethodsPlasmid pcDNA3-HPV16E6 was stably transfected into C33A cells by liposome, and the expression of HPV16E6 mRNA and protein in the transfected cells, the cells transfected with blank vector and the cells without transfection were identified by RT-PCR and Western blot analysis. The growth, proliferation and cell cycles of the 3 kinds of C33A cells we... |
| Keywords: | human papilloma virus cervical carcinoma cells proliferation cell cycles |
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