Neuropeptides can interfere with the recording properties of voltammetric carbon fibre electrodes.

The effects of neuropeptides on the recording properties of carbon fibre micro-electrodes used with differential pulse voltammetry were examined both in vitro and in vivo. The in vitro voltammetric signal recorded in a solution containing 3,4-dihydroxyphenylacetic acid (10(-4) M) was attenuated after the addition of thyrotropin releasing hormone, neuromedin N and neurotensin (10(-5) M). The administration of neurotensin (1 or 3 micrograms/microliters) into the nucleus accumbens adjacent to the carbon fibre electrode produced a decrease in the extracellular 3,4-dihydroxyphenylacetic acid peak. However, evidence was obtained that peptides alter the recording properties of the microelectrodes when present at sufficient concentrations and such an effect may explain the result obtained in vivo. In contrast, neurotensin administered into the ventral tegmental area increased the 3,4-dihydroxyphenylacetic acid peak in the nucleus accumbens as previously reported. Thus, attenuation of the voltammetric signal was caused by an effect of the peptide on the recording properties of the electrode rather than real diminution of the free oxidisable amine around the electrode. Caution should be taken when investigating the effects of peptides on extracellular amines or their metabolites using in vivo voltammetry with carbon fibre electrodes if the peptides are administered in high concentrations close to the electrode.