Early injection of OP-1 during distraction osteogenesis accelerates new bone formation in rabbits |
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| Authors: | Manuela Mandu-Hrit Tasima Haque Dominique Lauzier Maria Kotsiopriftis Frank Rauch Maryam Tabrizian |
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| Affiliation: | 1. Shriners Hospital, Montreal Children Hospital, Division of Orthopaedics, McGill University, Montreal, Que., Canada;2. Department of Biomedical Engineering, McGill University, Montreal, Que., Canada;3. Faculty of Dentistry, McGill University, Montreal, Que., Canada;4. Department of Biomedical Engineering, McGill University, Montreal, Que., Canada;5. Centre for Bone and Periodontal Research, McGill University, Montreal, Que., Canada |
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| Abstract: | Distraction osteogenesis (DO) is a surgical technique for generating new bone by applying controlled distraction of two bony segments post osteotomy. A limitation of the technique is the long time required for the new bone to consolidate. We investigated the effect of injecting osteogenic protein 1 (OP-1) at the beginning of distraction in a rabbit model of DO. Regenerate bone was evaluated using radiology, densitometry, micro-computed tomography (microCT) and histomorphometry. Immunohistochemsitry was used to evaluate changes in expression of various ligands, growth factors and receptors following OP-1 treatment. Compared to the control, a two-fold increase in bone volume was apparent for treated groups at 3 weeks post injection. An upregulation of almost all of the 41 genes examined was observed. Results suggested that applying OP-1 early during distraction can accelerate bone formation by the activation of numerous pathways. This study provides further insights on strategies to improve bone regeneration rate in DO. |
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| Keywords: | Distraction osteogenesis osteogenic protein-1 rabbits microCT immunohistochemistry |
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