Effects of Subchronic Exposures to Concentrated Ambient Particles in Mice: VI. Gene Expression in Heart and Lung Tissue |
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Authors: | Albert Gunnison Lung Chi Chen |
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Institution: | Nelson Institute of Environmental Medicine, Department of Environmental Medicine, New York University School of Medicine, Tuxedo, New York, USA |
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Abstract: | AbstractThe purpose of this exploratory study within the integrated subchronic inhalation exposure study () was to identify genes in heart and lung tissue that changed in expression level as a result of subchronic exposure to concentrated ambient particles (CAPs). Identification of CAPs exposure-related changes in gene expression could serve in the formulation of mechanistic hypotheses and/or to suggest possible biomarkers of exposure. In the exploratory study undertaken here, tissues from multiple replicates of ApoE/low-density-lipoprotein double knockout (DK) mice were examined for relative exposure-related changes in gene expression. Due to limited resources, the number of replicates was three for each tissue (lung and heart) of each exposure condition (CAPs or air control). A rigorous comparison of exposure versus control data using the“significance analysis of microarrays” (SAM) method indicated that only one gene was differentially expressed at a significant level. However, when using a less restrictive, nonstatistical analytical treatment of the data, several genes that might be involved in PM-related heart or lung pathology, and/or the circadian rhythm of physiological processes, were identified. A more comprehensive study is required to more definitively assess differences in gene expression in heart and lung resulting from exposure to CAPs. |
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