高效液相色谱法测定复方替硝唑漱口液中主药的含量

目的 :测定复方替硝唑漱口液中替硝唑和醋酸氯己定的含量。方法 :采用高效液相色谱法 ,以ODS为固定相 ,乙腈 -水(35∶65)为流动相 ,甲硝唑为内标,检测波长为254nm。结果 :替硝唑在0 01～0 10mg/ml浓度范围内线性关系良好(r=0 9999) ;醋酸氯己定在0 01～0 10mg/ml浓度范围内线性关系良好 (r=0 9997)。替硝唑和醋酸氯己定的平均回收率分别为97 06%～102 02 %、97 21%～103 40% ;日内差分别为0 20%～1 26 %、0 71 %～1 11% ,日间差分别为0 50 %～2 06 %、0 80%～2 17%。结论 :本法可用于测定替硝唑、醋酸氯己定在各种制剂中的含量。

Determination of the Contents of the Main Components in Co- tinidazole Gargle by HPLC

OBJECTIVE:To develop a HPLC method for determination of tinidazole and chlorhexidine acetate in co-tinidazole gargle.METHODS:Tinidazole and chlorhexidine acetate in co-tinidazole gargle were determined by RP-HPLC with metronidazole as the internal standard.ODS was adopted as stationary phase and acetonitrile-water(35∶65)as mobile phase.The detector was operated at UV254nm.RESULTS:The calibration curve of tinidazole was linear within the concenˉtration range of(0.01～0.10)mg/ml(r=0.9999);The calibration curve of chlorhexidine acetate was linear within the conˉcentration range of(0.01～0.10)mg/ml(r=0.9997).The average recoveries of tinidazole and chlorhexidine were(97.06～102.02)%and(97.21～103.40)%,respectively.The within-day coefficients of variance were(0.20～1.26)%and(0.71～1.11)%,respectively.The between-days coefficients of variance were(0.50～2.06)%and(0.80～2.17)%,respectiveˉly.CONCLUSION:The method is suitable for determination of preparations containing tinidazole and chlorhexidine acetate.