| 靶向mdr1基因转录小发夹RNA重组质粒的构建和序列分析 |
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| 引用本文： | 王萍玲,胡丽娜,张君,黄爱龙. 靶向mdr1基因转录小发夹RNA重组质粒的构建和序列分析[J]. 贵州医药, 2005, 29(1): 7-9 |
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| 作者姓名： | 王萍玲 胡丽娜 张君 黄爱龙 |
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| 作者单位： | 贵阳市妇幼保健院妇产科;重庆医科大学第二临床学院妇产科,400010;重庆医科大学病毒性肝炎研究所 |
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| 摘 要： | 目的 构建针对多药耐药基因(mdr1)编码区的发夹状RNA重组质粒栽体pshRNA-mdr1，并行序列分析，为下一步逆转肿瘤的多药耐药性打下基础。方法设计含19-21bp mdrl编码基因片段及中间以4个bp间隔的反向重复序列，经退火形成互补双链，克隆至转录栽体pTZU6 1上，转化JM109菌株，提取重组质粒酶切鉴定并序列分析。结果将合成的DNA片段成功克隆至载体上，经酶切及序列鉴定为目的序列。结论靶向mdr1基因发夹状RNA干扰重组质粒的成功构建，可进一步研究其对mdr1 mRNA转录的抑制，达到逆转肿瘤的多药耐药性的目的。
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| 关 键 词： | mdrl基因 小发夹RNA 重组质粒 序列分析 |
| 文章编号： | 1000-744X(2005)01-0007-03 |
Constructing and sequence analyzing of the transcriptional small hairpin RNA recombinant plasmid targeting mdr1 gene |
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| Wang Pingling,Hu Lina,Zhang Jun,et al.. Constructing and sequence analyzing of the transcriptional small hairpin RNA recombinant plasmid targeting mdr1 gene[J]. Guizhou Medical Journal, 2005, 29(1): 7-9 |
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| Authors: | Wang Pingling Hu Lina Zhang Jun et al. |
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| Affiliation: | Wang Pingling,Hu Lina,Zhang Jun,et al. Department of Obsterics and Gyne-clogy,the Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010 |
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| Abstract: | Objective To construct the recombinant plasmid targeting mdrl gene coding sequence pshRNA-mdrl and analyzing nucleic acid sequence. Methods A 19-21 bp reverse repeated motif of mdrl gene target sequence with 4bp spacer were designed and synthesized. Annealing and inserted into PT-ZU6+1. The recombinant plasmid transformed into JM109 strain. Then the recombinant plasmid was identified by restriction enzyme. Results The recombinant plasmid targeting mdrl gene was constructed successfully. Conclusion Constructing the pshRNA-mdrl recombinant plasmid helps to further study reversing multidrug resistance. |
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| Keywords: | mdrl gene shRNA Recombinant plasmid Sequence analyzing |
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